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Literature summary for 3.1.1.73 extracted from
- Expression of feruloyl esterase A from Aspergillus terreus and its application in biomass degradation (2015), Protein Expr. Purif., 115, 153-157.
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | the hydrolysis of corn stalk and corncob by xylanase from Aspergillus niger can be significantly improved in concert with recombinant FaeA | Aspergillus terreus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Aspergillus terreus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.29 | - |
sinapic acid methyl ester | pH 5.0, 40°C | Aspergillus terreus |  |
| 0.38 | - |
p-coumaric acid methyl ester | pH 5.0, 40°C | Aspergillus terreus | |
| 0.61 | - |
ferulic acid methyl ester | pH 5.0, 40°C | Aspergillus terreus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
- |
Aspergillus terreus |
| 37000 | - |
- |
Aspergillus terreus |
| 40000 | - |
- |
Aspergillus terreus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus terreus | A0A068FNL1 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Aspergillus terreus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ferulic acid methyl ester + H2O | - |
Aspergillus terreus | ferulic acid + methanol | - |
? | |
| p-coumaric acid methyl ester + H2O | - |
Aspergillus terreus | p-coumaric acid + methanol | - |
? | |
| sinapic acid methyl ester + H2O | - |
Aspergillus terreus | sinapic acid + methanol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000 and x * 37000, differently glycosylated forms, x * 27000, deglycosylated form | Aspergillus terreus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Aspergillus terreus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.31 | - |
p-coumaric acid methyl ester | pH 5.0, 40°C | Aspergillus terreus | |
| 0.94 | - |
sinapic acid methyl ester | pH 5.0, 40°C | Aspergillus terreus | |
| 2.6 | - |
ferulic acid methyl ester | pH 5.0, 40°C | Aspergillus terreus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Aspergillus terreus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 5.5 | more than 90% of maximum activity | Aspergillus terreus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.81 | - |
p-coumaric acid methyl ester | pH 5.0, 40°C | Aspergillus terreus | |
| 3.23 | - |
sinapic acid methyl ester | pH 5.0, 40°C | Aspergillus terreus | |
| 4.25 | - |
ferulic acid methyl ester | pH 5.0, 40°C | Aspergillus terreus | |
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Release 2023.1 (January 2023)
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