Literature summary for 3.1.1.73 extracted from

Li, J.; Pei, X.; Zhang, S.; Wu, Z.
Improving the thermostability of feruloyl esterase by DNA shuffling and site-directed mutagenesis (2015), Process Biochem., 50, 1783-1787.

No PubMed abstract available

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Protein Variants

Protein Variants	Comment	Organism
additional information	generation of a chimeric library from four homologous FAEs originated from fungi by a DNA shuffling strategy. Chimeras contain DNA fragments encoding the wild-type FaeA from Aspergillus terreus, FaeA from Penicillium chrysogenum and FaeA from Aspergillus flavus, as well as the M11 mutant of FaeA from Aspergillus niger. Chimeras with enhanced thermal stability contain segments from 3 or 4 of the parental enzymes. The half-lives of inactivation of the chimeras at 65°C are increased by up to 22 fold when compared to the most stable parental FAE. The release of ferulic acid from steam-exploded corn stalk using the best chimera is enhanced by 12.8fold when compared to the best parental FAE	Aspergillus niger
additional information	generation of a chimeric library from four homologous FAEs originated from fungi by a DNA shuffling strategy. Chimeras contain DNA fragments encoding the wild-type FaeA from Aspergillus terreus, FaeA from Penicillium chrysogenum and FaeA from Aspergillus flavus, as well as the M11 mutant of FaeA from Aspergillus niger. Chimeras with enhanced thermal stability contain segments from 3 or 4 of the parental enzymes. The half-lives of inactivation of the chimeras at 65°C are increased by up to 22 fold when compared to the most stable parental FAE. The release of ferulic acid from steam-exploded corn stalk using the best chimera is enhanced by 12.8fold when compared to the best parental FAE	Aspergillus terreus
additional information	generation of a chimeric library from four homologous FAEs originated from fungi by a DNA shuffling strategy. Chimeras contain DNA fragments encoding the wild-type FaeA from Aspergillus terreus, FaeA from Penicillium chrysogenum and FaeA from Aspergillus flavus, as well as the M11 mutant of FaeA from Aspergillus niger. Chimeras with enhanced thermal stability contain segments from 3 or 4 of the parental enzymes. The half-lives of inactivation of the chimeras at 65°C are increased by up to 22 fold when compared to the most stable parental FAE. The release of ferulic acid from steam-exploded corn stalk using the best chimera is enhanced by 12.8fold when compared to the best parental FAE	Penicillium chrysogenum
additional information	generation of a chimeric library from four homologous FAEs originated from fungi by a DNA shuffling strategy. Chimeras contain DNA fragments encoding the wild-type FaeA from Aspergillus terreus, FaeA from Penicillium chrysogenum and FaeA from Aspergillus flavus, as well as the M11 mutant of FaeA from Aspergillus niger. Chimeras with enhanced thermal stability contain segments from 3 or 4 of the parental enzymes. The half-lives of inactivation of the chimeras at 65°C are increased by up to 22 fold when compared to the most stable parental FAE. The release of ferulic acid from steam-exploded corn stalk using the best chimera is enhanced by 12.8fold when compared to the best parental FAE	Aspergillus flavus

Organism

Organism	UniProt	Comment	Textmining
Aspergillus flavus	R9UUB4	-	-
Aspergillus niger	O42807	-	-
Aspergillus terreus	Q0CBM7	-	-
Aspergillus terreus NIH 2624	Q0CBM7	-	-
Penicillium chrysogenum	S5Q8E1	-	-

Synonyms

Synonyms	Comment	Organism
FAEA	-	Aspergillus niger
FAEA	-	Aspergillus terreus
FAEA	-	Aspergillus flavus
XM 001217492	-	Aspergillus terreus

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Release 2023.1 (January 2023)