Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Schizophyllum commune |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.044 | - |
methyl 4-hydroxybenzoate | pH 7.5, 37°C | Schizophyllum commune | |
0.059 | - |
methyl 4-hydroxybenzoate | pH 7.5, 37°C | Schizophyllum commune | |
0.114 | - |
chlorogenic acid | pH 7.5, 37°C | Schizophyllum commune | |
0.123 | - |
methyl caffeate | pH 7.5, 37°C | Schizophyllum commune | |
0.124 | - |
methyl caffeate | pH 7.5, 37°C | Schizophyllum commune | |
0.131 | - |
chlorogenic acid | pH 7.5, 37°C | Schizophyllum commune | |
0.137 | - |
methyl p-coumarate | pH 7.5, 37°C | Schizophyllum commune | |
0.147 | - |
methyl ferulate | pH 7.5, 37°C | Schizophyllum commune | |
0.151 | - |
methyl p-coumarate | pH 7.5, 37°C | Schizophyllum commune | |
0.16 | - |
methyl ferulate | pH 7.5, 37°C | Schizophyllum commune |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizophyllum commune | D8QHC3 | - |
- |
Schizophyllum commune | D8QHD6 | - |
- |
Schizophyllum commune H4-8 | D8QHC3 | - |
- |
Schizophyllum commune H4-8 | D8QHD6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-O-trans-feruloyl-L-arabinofuranose + H2O | 88% of the activity with methyl p-coumarate | Schizophyllum commune | L-arabinofuranose + ferulate | - |
? | |
5-O-trans-feruloyl-L-arabinofuranose + H2O | 90% of the activity with methyl p-coumarate | Schizophyllum commune | L-arabinofuranose + ferulate | - |
? | |
alpha-L-galactopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose + H2O | 93% of the activity with methyl p-coumarate | Schizophyllum commune | alpha-L-galactopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->2)-L-arabinofuranose + ferulate | - |
? | |
beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose + H2O | 96% of the activity with methyl p-coumarate | Schizophyllum commune | beta-D-xylopyranosyl-(1->2)-L-arabinofuranose + ferulate | - |
? | |
beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose + H2O | 97% of the activity with methyl p-coumarate | Schizophyllum commune | beta-D-xylopyranosyl-(1->2)-L-arabinofuranose + ferulate | - |
? | |
chlorogenic acid + H2O | 11% of the activity with methyl p-coumarate | Schizophyllum commune | caffeate + quinate | - |
? | |
chlorogenic acid + H2O | 11% of the activity with methyl p-coumarate | Schizophyllum commune H4-8 | caffeate + quinate | - |
? | |
coffee pulp + H2O | - |
Schizophyllum commune | ferulic acid + caffeic acid + p-coumaric acid + ? | - |
? | |
D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->3)-5-O-trans-feruloyl-L-arabinofuranose + H2O | 94% of the activity with methyl p-coumarate | Schizophyllum commune | D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->3)-L-arabinofuranose + ferulate | - |
? | |
D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->3)-5-O-trans-feruloyl-L-arabinofuranose + H2O | 95% of the activity with methyl p-coumarate | Schizophyllum commune | D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->3)-L-arabinofuranose + ferulate | - |
? | |
destarched wheat bran + H2O | - |
Schizophyllum commune | ferulic acid + ? | - |
? | |
destarched wheat bran + H2O | - |
Schizophyllum commune H4-8 | ferulic acid + ? | - |
? | |
ethyl ferulate + H2O | 62% of the activity with methyl p-coumarate | Schizophyllum commune | ethanol + ferulate | - |
? | |
ethyl ferulate + H2O | 70% of the activity with methyl p-coumarate | Schizophyllum commune | ethanol + ferulate | - |
? | |
methyl 4-hydroxybenzoate + H2O | 27% of the activity with methyl p-coumarate | Schizophyllum commune | methanol + 4-hydroxybenzoate | - |
? | |
methyl 4-hydroxybenzoate + H2O | 31% of the activity with methyl p-coumarate | Schizophyllum commune | methanol + 4-hydroxybenzoate | - |
? | |
methyl 4-hydroxybenzoate + H2O | 27% of the activity with methyl p-coumarate | Schizophyllum commune H4-8 | methanol + 4-hydroxybenzoate | - |
? | |
methyl 4-hydroxybenzoate + H2O | 31% of the activity with methyl p-coumarate | Schizophyllum commune H4-8 | methanol + 4-hydroxybenzoate | - |
? | |
methyl caffeate + H2O | 47% of the activity with methyl p-coumarate | Schizophyllum commune | methanol + caffeate | - |
? | |
methyl caffeate + H2O | 50% of the activity with methyl p-coumarate | Schizophyllum commune | methanol + caffeate | - |
? | |
methyl ferulate + H2O | 65% of the activity with methyl p-coumarate | Schizophyllum commune | methanol + ferulate | - |
? | |
methyl ferulate + H2O | 72% of the activity with methyl p-coumarate | Schizophyllum commune | methanol + ferulate | - |
? | |
methyl p-coumarate + H2O | - |
Schizophyllum commune | methanol + p-coumarate | - |
? | |
additional information | enzyme preferably hydrolyzes feruloylated saccharides with ferulic acid esterified to the O-5 position of arabinose residues and shows an ability to hydrolyze benzoic acid esters | Schizophyllum commune | ? | - |
? | |
additional information | enzyme preferably hydrolyzes feruloylated saccharides with ferulic acid esterified to the O-5 position of arabinose residues and shows an ability to hydrolyze benzoic acid esters | Schizophyllum commune H4-8 | ? | - |
? | |
sugar beet pectin + H2O | - |
Schizophyllum commune | ferulic acid + ? | - |
? | |
sugar beet pectin + H2O | - |
Schizophyllum commune H4-8 | ferulic acid + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FaeD1 | - |
Schizophyllum commune |
FaeD2 | - |
Schizophyllum commune |
SCHCODRAFT_60993 | - |
Schizophyllum commune |
SCHCODRAFT_61055 | - |
Schizophyllum commune |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.6 | - |
chlorogenic acid | pH 7.5, 37°C | Schizophyllum commune | |
7.5 | - |
chlorogenic acid | pH 7.5, 37°C | Schizophyllum commune | |
11.2 | - |
methyl 4-hydroxybenzoate | pH 7.5, 37°C | Schizophyllum commune | |
12.5 | - |
methyl 4-hydroxybenzoate | pH 7.5, 37°C | Schizophyllum commune | |
34.1 | - |
methyl caffeate | pH 7.5, 37°C | Schizophyllum commune | |
38.4 | - |
methyl caffeate | pH 7.5, 37°C | Schizophyllum commune | |
51 | - |
methyl ferulate | pH 7.5, 37°C | Schizophyllum commune | |
54.7 | - |
methyl ferulate | pH 7.5, 37°C | Schizophyllum commune | |
91.4 | - |
methyl p-coumarate | pH 7.5, 37°C | Schizophyllum commune | |
105.1 | - |
methyl p-coumarate | pH 7.5, 37°C | Schizophyllum commune |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
57.3 | - |
chlorogenic acid | pH 7.5, 37°C | Schizophyllum commune | |
58.2 | - |
chlorogenic acid | pH 7.5, 37°C | Schizophyllum commune | |
210 | - |
methyl 4-hydroxybenzoate | pH 7.5, 37°C | Schizophyllum commune | |
257.2 | - |
methyl 4-hydroxybenzoate | pH 7.5, 37°C | Schizophyllum commune | |
275.1 | - |
methyl caffeate | pH 7.5, 37°C | Schizophyllum commune | |
310.6 | - |
methyl caffeate | pH 7.5, 37°C | Schizophyllum commune | |
319.2 | - |
methyl ferulate | pH 7.5, 37°C | Schizophyllum commune | |
372.6 | - |
methyl ferulate | pH 7.5, 37°C | Schizophyllum commune | |
666.2 | - |
methyl p-coumarate | pH 7.5, 37°C | Schizophyllum commune | |
696.3 | - |
methyl p-coumarate | pH 7.5, 37°C | Schizophyllum commune |