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Literature summary for 3.1.1.73 extracted from

  • Dotsenko, G.; Tong, X.; Pilgaard, B.; Busk, P.; Lange, L.
    Acidic-alkaline ferulic acid esterase from Chaetomium thermophilum var. dissitum: Molecular cloning and characterization of recombinant enzyme expressed in Pichia pastoris (2016), Biocatal. Agricult. Biotechnol., 5, 48-55.
No PubMed abstract available
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 methyl p-coumarate pH 6.0, 55°C Thermochaetoides dissita
0.025
-
 ethyl ferulate pH 6.0, 55°C Thermochaetoides dissita
0.031
-
 methyl caffeate pH 6.0, 55°C Thermochaetoides dissita
0.25
-
 1-naphthyl acetate pH 6.0, 55°C Thermochaetoides dissita
0.54
-
 4-nitrophenyl butanoate pH 6.0, 55°C Thermochaetoides dissita

Organism

Organism UniProt Comment Textmining
Thermochaetoides dissita
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl acetate + H2O
-
 Thermochaetoides dissita 1-naphthol + acetic acid
-
 ?
4-nitrophenyl butanoate + H2O
-
 Thermochaetoides dissita 4-nitrophenol + butanoic acid
-
 ?
arabinoxylan + H2O
-
 Thermochaetoides dissita ferulic acid + ? enzyme releases ferulic acid from wheat insoluble arabinoxylan ?
ethyl ferulate + H2O
-
 Thermochaetoides dissita ethanol + ferulic acid
-
 ?
methyl caffeate + H2O
-
 Thermochaetoides dissita methanol + caffeic acid
-
 ?
methyl p-coumarate + H2O
-
 Thermochaetoides dissita methanol + p-coumarate
-
 ?
rye grass + H2O
-
 Thermochaetoides dissita ferulic acid + p-coumaric acid + ? enzyme releases ferulic acid and p-coumaric acid from rye grass ?
wheat straw + H2O
-
 Thermochaetoides dissita ferulic acid + p-coumaric acid + ? enzyme releases ferulic acid and p-coumaric acid from wheat straw ?

Synonyms

Synonyms Comment Organism
FAE
-
 Thermochaetoides dissita

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Thermochaetoides dissita

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 65 more than 70% of maximum activity Thermochaetoides dissita

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 24 h, 99% residual activity Thermochaetoides dissita
60
-
 24 h, 73% residual activity Thermochaetoides dissita

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1
-
 methyl caffeate pH 6.0, 55°C Thermochaetoides dissita
4.1
-
 ethyl ferulate pH 6.0, 55°C Thermochaetoides dissita
5.8
-
 methyl p-coumarate pH 6.0, 55°C Thermochaetoides dissita
24
-
 4-nitrophenyl butanoate pH 6.0, 55°C Thermochaetoides dissita
32
-
 1-naphthyl acetate pH 6.0, 55°C Thermochaetoides dissita

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Thermochaetoides dissita

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 24 h, more than 70% residual activity Thermochaetoides dissita

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
99
-
 methyl caffeate pH 6.0, 55°C Thermochaetoides dissita
109
-
 4-nitrophenyl butanoate pH 6.0, 55°C Thermochaetoides dissita
128
-
 1-naphthyl acetate pH 6.0, 55°C Thermochaetoides dissita
164
-
 ethyl ferulate pH 6.0, 55°C Thermochaetoides dissita
363
-
 methyl p-coumarate pH 6.0, 55°C Thermochaetoides dissita