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Literature summary for 3.1.1.73 extracted from

  • Qi, M.; Wang, P.; Selinger, L.B.; Yanke, L.J.; Forster, R.J.; McAllister, T.A.
    Isolation and characterization of a ferulic acid esterase (Fae1A) from the rumen fungus Anaeromyces mucronatus (2011), J. Appl. Microbiol., 110, 1341-1350.
    View publication on PubMed
Search for more literature for 3.1.1.73

Cloned(Commentary)

Cloned (Comment) Organism
gene fae1A, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21(DE3) Anaeromyces mucronatus

Inhibitors

Inhibitors Comment Organism Structure
additional information Fae1A is not affected by the addition of EDTA Anaeromyces mucronatus
phenylmethylsulfonyl fluoride
-
 Anaeromyces mucronatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0052
-
 ethyl ferulate pH 7.2, 37°C Anaeromyces mucronatus
0.0067
-
 methyl ferulate pH 7.2, 37°C Anaeromyces mucronatus

Organism

Organism UniProt Comment Textmining
Anaeromyces mucronatus F2YCB6 gene fae1A
-
Anaeromyces mucronatus YE505 F2YCB6 gene fae1A
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-naphthyl acetate + H2O
-
 Anaeromyces mucronatus 2-naphthol + acetate
-
 ?
2-naphthyl acetate + H2O
-
 Anaeromyces mucronatus YE505 2-naphthol + acetate
-
 ?
ethyl ferulate + H2O
-
 Anaeromyces mucronatus ferulic acid + ethanol
-
 ?
ethyl ferulate + H2O
-
 Anaeromyces mucronatus YE505 ferulic acid + ethanol
-
 ?
methyl ferulate + H2O
-
 Anaeromyces mucronatus ferulic acid + methanol
-
 ?
methyl ferulate + H2O
-
 Anaeromyces mucronatus YE505 ferulic acid + methanol
-
 ?
additional information Fae1A exhibist activity against a number of substrates including naphthyl fatty acid esters, 4nitrophenyl fatty acid esters and hydroxylcinnamic acid esters, it has a higher affinity for ethyl and methyl ferulate than for the acetyl esters. It releases ferulic acid and 4-coumaric acid from barley straw. A serine residue plays a role in its activity Anaeromyces mucronatus ?
-
 ?
additional information Fae1A exhibist activity against a number of substrates including naphthyl fatty acid esters, 4nitrophenyl fatty acid esters and hydroxylcinnamic acid esters, it has a higher affinity for ethyl and methyl ferulate than for the acetyl esters. It releases ferulic acid and 4-coumaric acid from barley straw. A serine residue plays a role in its activity Anaeromyces mucronatus YE505 ?
-
 ?

Synonyms

Synonyms Comment Organism
Fae1A
-
 Anaeromyces mucronatus
ferulic acid esterase
-
 Anaeromyces mucronatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Anaeromyces mucronatus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 37 activity is optimal at 37°C, but 60% of activity is retained at 15°C. No activity is observed at 45°C Anaeromyces mucronatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Anaeromyces mucronatus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 over 50% activity within this range Anaeromyces mucronatus