Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | ferulic acid release by recombinant FaeB or FaeC is increased considerably by 17.6fold and 16fold, respectively in the presence of Thermomyces lanuginosus xylanase | Aspergillus oryzae |
Cloned (Comment) | Organism |
---|---|
PCR product cloned into the pGEM-T Easy vector. FaeB and FaeC fragments subcloned into EcoRI-XbaI-digested pPICZalphaA expression vector. FaeB and FaeC expressed in Pichia pastoris GS115 by fusing to the Saccharomyces cerevisiae alpha-factor secretion signal peptide | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 61000, purified recombinant FaeB, SDS-PAGE. x * 75000, purified recombinant FaeC, SDS-PAGE. x * 55000, purified recombinant FaeB and FaeC after N-deglycosylation with endo-H, SDS-PAGE | Aspergillus oryzae |
61000 | - |
x * 61000, purified recombinant FaeB, SDS-PAGE. x * 75000, purified recombinant FaeC, SDS-PAGE. x * 55000, purified recombinant FaeB and FaeC after N-deglycosylation with endo-H, SDS-PAGE | Aspergillus oryzae |
75000 | - |
x * 61000, purified recombinant FaeB, SDS-PAGE. x * 75000, purified recombinant FaeC, SDS-PAGE. x * 55000, purified recombinant FaeB and FaeC after N-deglycosylation with endo-H, SDS-PAGE | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Aspergillus oryzae RIB 40 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
FaeB and FaeC, in a two-step procedure using anion-exchange chromatography and gel filtration | Aspergillus oryzae |
Subunits | Comment | Organism |
---|---|---|
? | x * 61000, purified recombinant FaeB, SDS-PAGE. x * 75000, purified recombinant FaeC, SDS-PAGE. x * 55000, purified recombinant FaeB and FaeC after N-deglycosylation with endo-H, SDS-PAGE | Aspergillus oryzae |
Synonyms | Comment | Organism |
---|---|---|
FAEB | - |
Aspergillus oryzae |
FaeC | - |
Aspergillus oryzae |
feruloyl esterase | - |
Aspergillus oryzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
purified recombinant FaeB and FaeC retain more than 90% of their activity after incubation at 55°C for 30 min. FaeC retains 40% of its activity after treatment at 65°C, whereas FaeB almost loses its activity | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
purified recombinant FaeB and FaeC | Aspergillus oryzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | purified recombinant FaeB and FaeC show more than 90% of their maximum activity | Aspergillus oryzae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | purified recombinant FaeB | Aspergillus oryzae |
7 | 10 | purified recombinant FaeC | Aspergillus oryzae |