Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.73 extracted from

  • Kancuchi, M.; Watanabe, S.; Tsukada, T.; Atta, K.; Kakuta, T.; Koizumi, T.
    Purification and characteristics of feruloyl esterase from Aspergillus awamori G-2 strain (2008), J. Food Sci., 73, C458-C463.
    View publication on PubMed
Search for more literature for 3.1.1.73

Application

Application Comment Organism
food industry Aspergillus awamori is applicable to food production and Awamori production. Aspergillus awamori
food industry the enzyme shows stable activity at pH 3 and 50°C, thus the enzyme is acid tolerant, but not heat tolerant, making this enzyme useful for food production Aspergillus awamori

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 1 mM inhibits 10% of the activity Aspergillus awamori
Cu2+ 1 mM inhibits 20% of the activity Aspergillus awamori
diisopropyl fluorophosphate inhibits completely Aspergillus awamori
diisopropyl fluorophosphates inhibits feruloyl esterase activity completely Aspergillus awamori
Fe2+ 1 mM inhibits 80% of the activity; inhibits 80% of the activity Aspergillus awamori
Hg2+ 1 mM inhibits completely; inhibits feruloyl esterase activity completely Aspergillus awamori
Mn2+ 1 mM inhibits 10% of the activity Aspergillus awamori
Monoiodoacetic acid 1 mM inhibits 5% of the activity Aspergillus awamori
additional information 1 mM Ag2+, Ca2+, Mg2+, Zn2+, EDTA and SDS have no effect on activity Aspergillus awamori
Ni2+ 1 mM inhibits 5% of the activity Aspergillus awamori
p-chloromercuribenzoic acid 1 mM inhibits 10% of the activity Aspergillus awamori
phenylmethylsulfonyl fluoride 1 mM inhibits completely; inhibits feruloyl esterase activity completely Aspergillus awamori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 1-naphthyl acetate in 20% N,N-dimethyl formamide, 50 mM acetate buffer, pH 5, with 0.05 ml enzyme solution, at 37°C for 10 min Aspergillus awamori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
 size-exclusion chromatography Aspergillus awamori

Organism

Organism UniProt Comment Textmining
Aspergillus awamori
-
-
-
Aspergillus awamori G-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by ion-exchange chromatography, gel filtration and ammonium sulfate precipitation Aspergillus awamori
precipitation, ion-exchange chromatography, size-exclusion chromatography, and HPLC chromatography Aspergillus awamori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl acetate + H2O highest activity Aspergillus awamori acetic acid + 1-naphthol
-
 ?
1-naphthyl acetate + H2O 100% relative activity Aspergillus awamori acetic acid + 1-naphthol
-
 ?
1-naphthyl acetate + H2O highest activity Aspergillus awamori G-2 acetic acid + 1-naphthol
-
 ?
1-naphthyl acetate + H2O 100% relative activity Aspergillus awamori G-2 acetic acid + 1-naphthol
-
 ?
1-naphthyl butyrate + H2O 5% of the activity related to 1-naphthyl acetate Aspergillus awamori butyric acid + 1-naphthol
-
 ?
1-naphthyl butyrate + H2O 5% of the activity compared to 1-naphthyl acetate Aspergillus awamori butyric acid + 1-naphthol
-
 ?
1-naphthyl propionate + H2O 40% of the activity related to 1-naphthyl acetate Aspergillus awamori propionic acid + 1-naphthol
-
 ?
1-naphthyl propionate + H2O 40% of the activity compared to 1-naphthyl acetate Aspergillus awamori propionic acid + 1-naphthol
-
 ?
1-naphthyl propionate + H2O 40% of the activity related to 1-naphthyl acetate Aspergillus awamori G-2 propionic acid + 1-naphthol
-
 ?
additional information The activity of feruloyl esterase is decreased against substrates containing more than 3 carbons; 2-naphthyl acetate does not react completely Aspergillus awamori ?
-
 ?
additional information 2-naphthyl acetate does not react completely. No adsorption of the feruloyl esterase to a cell wall. Xylan and starch do not absorb the enzyme but it is absorbed by cellulose Aspergillus awamori ?
-
 ?
additional information The activity of feruloyl esterase is decreased against substrates containing more than 3 carbons; 2-naphthyl acetate does not react completely Aspergillus awamori G-2 ?
-
 ?
additional information 2-naphthyl acetate does not react completely. No adsorption of the feruloyl esterase to a cell wall. Xylan and starch do not absorb the enzyme but it is absorbed by cellulose Aspergillus awamori G-2 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 78000, SDS-PAGE, size-exclusion chromatography Aspergillus awamori
monomer 1 * 78000, SDS-PAGE or gel filtration Aspergillus awamori

Synonyms

Synonyms Comment Organism
feruloyl esterase
-
 Aspergillus awamori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Aspergillus awamori

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50
-
 Aspergillus awamori

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 50
-
 Aspergillus awamori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Aspergillus awamori

pH Range

pH Minimum pH Maximum Comment Organism
3 5
-
 Aspergillus awamori

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
 The feruloyl esterase from Aspergillus awamori G-2 is more stable under the acid condition than that of the other black Aspergillus group. Aspergillus awamori
3 5
-
 Aspergillus awamori