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Literature summary for 3.1.1.73 extracted from

  • Wong, D.W.
    Feruloyl esterase: a key enzyme in biomass degradation (2006), Appl. Biochem. Biotechnol., 133, 87-112.
    View publication on PubMed
Search for more literature for 3.1.1.73

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 O-[5-O-(transferuloyl)-alpha-L-arabinofuranosyl]-(1,3)-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose 37°C, pH 6.0 Piromyces sp. 'equi'
0.007
-
 methyl coumarate 37°C, pH 6.0 Talaromyces funiculosus
0.01
-
 O-beta-D-xylopyranosyl-(1,4)-O-[5-O-(trans-feruloyl)-alpha-L-arabinofuranosyl-(1,3)]-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose ferulate 40°C, pH 7.0, isoenzyme FAE-II Neocallimastix sp.
0.02
-
 methyl caffeate 37°C, pH 6.0 Neurospora crassa
0.021
-
 methyl coumarate 37°C, pH 6.0 Neurospora crassa
0.032
-
 O-beta-D-xylopyranosyl-(1,4)-O-[5-O-(trans-feruloyl)-alpha-L-arabinofuranosyl-(1,3)]-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose ferulate 40°C, pH 6.2, isoenzyme FAE-I Neocallimastix sp.
0.047
-
 methyl ferulate 37°C, pH 6.0 Talaromyces funiculosus
0.075
-
 5-O-(trans-feruloyl)-L-arabinofuranose 37°C, pH 6.0 Piromyces sp. 'equi'
0.139
-
 methyl caffeate 37°C, pH 6.0 Talaromyces funiculosus
0.2
-
 methyl coumarate 45°C, pH 6.0, isoenzyme FAE-I Fusarium oxysporum
0.25
-
 methyl ferulate 37°C, pH 6.0 Neurospora crassa
0.26
-
 O-beta-D-xylopyranosyl-(1,4)-O-[5-O-(trans-feruloyl)-alpha-L-arabinofuranosyl]-(1,3)-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose
-
 Pseudomonas fluorescens
0.26
-
 methyl caffeate 45°C, pH 6.0, isoenzyme FAE-I Fusarium oxysporum
0.29
-
 methyl sinapate 45°C, pH 6.0, isoenzyme FAE-II Fusarium oxysporum
0.46
-
 O-[2-O-(transferuloyl)-alpha-L-arabinofuranosyl]-(1,5)-L-arabinose 37°C, pH 6.0 Neurospora crassa
0.47
-
 methyl p-coumarate
-
 Aspergillus niger
0.56
-
 O-[5-O-(trans-feruloyl)-alpha-L-arabinofuranosyl]-(1,3)-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose
-
 Pseudomonas fluorescens
0.58
-
 methyl ferulate 45°C, pH 6.0, isoenzyme FAE-II Fusarium oxysporum
0.6
-
 methyl ferulate 45°C, pH 6.0, isoenzyme FAE-I Fusarium oxysporum
0.68
-
 methyl coumarate 45°C, pH 6.0, isoenzyme FAE-II Fusarium oxysporum
0.81
-
 methyl caffeate 45°C, pH 6.0, isoenzyme FAE-II Fusarium oxysporum
0.84
-
 5-O-(trans-feruloyl)-L-arabinofuranose
-
 Pseudomonas fluorescens
1.11
-
 methyl ferulate pH 5.3, 50°C Aspergillus niger
1.12
-
 methyl sinapate 45°C, pH 6.0, isoenzyme FAE-I Fusarium oxysporum
1.2
-
 methyl sinapate
-
 Aspergillus niger
1.86
-
 methyl ferulate 30°C, pH 5.5 Streptomyces olivochromogenes
2.1
-
 methyl ferulate
-
 Pseudomonas fluorescens
2.6
-
 methyl ferulate 37°C, pH 6.0 Penicillium expansum
2.9
-
 methyl coumarate 37°C, pH 6.0 Penicillium expansum
5
-
 O-beta-D-xylopyranosyl-(1,4)-O-[5-O-(trans-feruloyl)-alpha-L-arabinofuranosyl-(1,3)]-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose 60°C, pH 6.0 Acetivibrio thermocellus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 isoenzyme FAE-II Neocallimastix sp.
27000
-
 isoenzyme FAE-II Fusarium oxysporum
29000
-
-
 Streptomyces olivochromogenes
29000
-
-
 Aspergillus niger
30000
-
-
 Aspergillus oryzae
31000
-
 isoenzyme FAE-I Fusarium oxysporum
35000
-
-
 Aspergillus awamori
35000
-
-
 Neurospora crassa
35500
-
-
 Talaromyces funiculosus
55500
-
-
 Piromyces sp. 'equi'
57500
-
-
 Penicillium expansum
58500
-
-
 Pseudomonas fluorescens
69000
-
 isoenzyme FAE-I Neocallimastix sp.
90000
-
-
 Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-
Aspergillus awamori
-
-
-
Aspergillus niger O42807
-
-
Aspergillus oryzae
-
-
-
Fusarium oxysporum
-
-
-
Neocallimastix sp.
-
-
-
Neurospora crassa Q9HGR3
-
-
Penicillium expansum
-
-
-
Piromyces sp. 'equi' Q9Y871
-
-
Pseudomonas fluorescens
-
 supsp. cellulosa
-
Streptomyces olivochromogenes
-
-
-
Talaromyces funiculosus Q9HE18
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-O-(trans-feruloyl)-L-arabinofuranose + H2O
-
 Pseudomonas fluorescens ferulic acid + L-arabinofuranose
-
 ?
5-O-(trans-feruloyl)-L-arabinofuranose + H2O
-
 Piromyces sp. 'equi' ?
-
 ?
methyl caffeate + H2O isoenzyme FAE-I Fusarium oxysporum caffeic acid + methanol
-
 ?
methyl caffeate + H2O
-
 Neurospora crassa caffeate + methanol
-
 ?
methyl caffeate + H2O
-
 Talaromyces funiculosus caffeate + methanol
-
 ?
methyl caffeate + H2O isoenzyme FAE-II Fusarium oxysporum caffeate + methanol
-
 ?
methyl coumarate + H2O
-
 Penicillium expansum coumaric acid + methanol
-
 ?
methyl coumarate + H2O
-
 Neurospora crassa coumaric acid + methanol
-
 ?
methyl coumarate + H2O
-
 Talaromyces funiculosus coumaric acid + methanol
-
 ?
methyl coumarate + H2O isoenzyme FAE-I Fusarium oxysporum coumaric acid + methanol
-
 ?
methyl coumarate + H2O isoenzyme FAE-II Fusarium oxysporum coumaric acid + methanol
-
 ?
methyl ferulate + H2O
-
 Pseudomonas fluorescens ferulic acid + methanol
-
 ?
methyl ferulate + H2O
-
 Streptomyces olivochromogenes ferulic acid + methanol
-
 ?
methyl ferulate + H2O
-
 Penicillium expansum ferulic acid + methanol
-
 ?
methyl ferulate + H2O
-
 Neurospora crassa ferulic acid + methanol
-
 ?
methyl ferulate + H2O
-
 Aspergillus niger ferulic acid + methanol
-
 ?
methyl ferulate + H2O
-
 Talaromyces funiculosus ferulic acid + methanol
-
 ?
methyl ferulate + H2O isoenzyme FAE-I Fusarium oxysporum ferulic acid + methanol
-
 ?
methyl ferulate + H2O isoenzyme FAE-II Fusarium oxysporum ferulic acid + methanol
-
 ?
methyl p-coumarate + H2O
-
 Aspergillus niger p-coumaric acid + methanol
-
 ?
methyl sinapate + H2O
-
 Aspergillus niger sinapic acid + methanol
-
 ?
methyl sinapate + H2O isoenzyme FAE-I Fusarium oxysporum sinapic acid + methanol
-
 ?
methyl sinapate + H2O isoenzyme FAE-II Fusarium oxysporum sinapic acid + methanol
-
 ?
O-beta-D-xylopyranosyl-(1,4)-O-[5-O-(trans-feruloyl)-alpha-L-arabinofuranosyl-(1,3)]-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose + H2O
-
 Pseudomonas fluorescens O-beta-D-xylopyranosyl-(1,4)-O-alpha-L-arabinofuranosyl-(1,3)-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose + trans-ferulic acid
-
 ?
O-beta-D-xylopyranosyl-(1,4)-O-[5-O-(trans-feruloyl)-alpha-L-arabinofuranosyl-(1,3)]-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose + H2O
-
 Acetivibrio thermocellus O-beta-D-xylopyranosyl-(1,4)-O-alpha-L-arabinofuranosyl-(1,3)-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose + trans-ferulic acid
-
 ?
O-beta-D-xylopyranosyl-(1,4)-O-[5-O-(trans-feruloyl)-alpha-L-arabinofuranosyl-(1,3)]-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose + H2O
-
 Neocallimastix sp. O-beta-D-xylopyranosyl-(1,4)-O-alpha-L-arabinofuranosyl-(1,3)-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose + trans-ferulic acid
-
 ?
O-beta-D-xylopyranosyl-(1,4)-O-[5-O-(trans-feruloyl)-alpha-L-arabinofuranosyl-(1,3)]-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose ferulate + H2O
-
 Neocallimastix sp. O-beta-D-xylopyranosyl-(1,4)-O-[5-O-(trans-feruloyl)-alpha-L-arabinofuranosyl-(1,3)]-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose + ferulate
-
 ?
O-[2-O-(trans-feruloyl)-alpha-L-arabinofuranosyl]-(1,5)-L-arabinose + H2O
-
 Neurospora crassa trans-ferulate + alpha-L-arabinofuranosyl-(1->5)-L-arabinose
-
 ?
O-[5-O-(trans-feruloyl)-alpha-L-arabinofuranosyl]-(1,3)-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose + H2O
-
 Pseudomonas fluorescens alpha-L-arabinofuranosyl-(1,3)-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose + trans-ferulic acid
-
 ?
O-[5-O-(trans-feruloyl)-alpha-L-arabinofuranosyl]-(1->3)-O-beta-D-xylopyranosyl-(1->4)-D-xylopyranose + H2O
-
 Piromyces sp. 'equi' trans-ferulate + alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylopyranose
-
 ?

Synonyms

Synonyms Comment Organism
AnFaeA
-
 Aspergillus niger
EstA
-
 Piromyces sp. 'equi'
esterase A
-
 Piromyces sp. 'equi'
FAE-B
-
 Neurospora crassa
FAE-B
-
 Talaromyces funiculosus
FAE-I
-
 Fusarium oxysporum
FAE-I
-
 Neocallimastix sp.
FAE-II
-
 Fusarium oxysporum
FAE-II
-
 Neocallimastix sp.
FAEB
-
 Neurospora crassa
FAEB
-
 Talaromyces funiculosus
ferulic acid esterase A
-
 Aspergillus niger
ferulic acid esterase B
-
 Neurospora crassa
ferulic acid esterase B
-
 Talaromyces funiculosus
ferulic acid esterase B
-
 Piromyces sp. 'equi'
feruloyl esterase A
-
 Aspergillus niger
feruloyl esterase B
-
 Piromyces sp. 'equi'
XYLD
-
 Pseudomonas fluorescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Streptomyces olivochromogenes
37
-
-
 Penicillium expansum
45
-
-
 Aspergillus oryzae
45
-
-
 Aspergillus awamori
45
-
-
 Acetivibrio thermocellus
45
-
 isoenzyme FAE-II Fusarium oxysporum
50 60
-
 Piromyces sp. 'equi'
55
-
-
 Neurospora crassa
55
-
 isoenzyme FAE-I Fusarium oxysporum
55 60
-
 Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 stable up to, isoenzyme FAE-I Fusarium oxysporum
30 70 stable Piromyces sp. 'equi'
45
-
 stable up to, isoenzyme FAE-II Fusarium oxysporum
50
-
 stable up to Aspergillus awamori
55
-
 stable up to Neurospora crassa
70
-
 stable up to Acetivibrio thermocellus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 O-[2-O-(transferuloyl)-alpha-L-arabinofuranosyl]-(1,5)-L-arabinose 37°C, pH 6.0 Neurospora crassa
additional information
-
 methyl caffeate 45°C, pH 6.0, isoenzyme FAE-I Fusarium oxysporum
0.41
-
 methyl ferulate 37°C, pH 6.0 Neurospora crassa
0.41
-
 methyl coumarate 37°C, pH 6.0 Neurospora crassa
0.47
-
 methyl sinapate 45°C, pH 6.0, isoenzyme FAE-I Fusarium oxysporum
3.5
-
 methyl coumarate 45°C, pH 6.0, isoenzyme FAE-I Fusarium oxysporum
4.8
-
 methyl caffeate 37°C, pH 6.0 Neurospora crassa
5.92
-
 methyl caffeate 45°C, pH 6.0, isoenzyme FAE-I Fusarium oxysporum
6.04
-
 O-[2-O-(transferuloyl)-alpha-L-arabinofuranosyl]-(1,5)-L-arabinose 37°C, pH 6.0 Neurospora crassa
6.85
-
 methyl ferulate 45°C, pH 6.0, isoenzyme FAE-I Fusarium oxysporum
7.9
-
 methyl caffeate 45°C, pH 6.0, isoenzyme FAE-II Fusarium oxysporum
12.19
-
 methyl ferulate 37°C, pH 6.0 Neurospora crassa
12.19
-
 methyl coumarate 37°C, pH 6.0 Neurospora crassa
13
-
 methyl coumarate 45°C, pH 6.0, isoenzyme FAE-II Fusarium oxysporum
16
-
 methyl ferulate 37°C, pH 6.0 Talaromyces funiculosus
19.88
-
 methyl coumarate 45°C, pH 6.0, isoenzyme FAE-I Fusarium oxysporum
24.1
-
 methyl caffeate 37°C, pH 6.0 Talaromyces funiculosus
24.2
-
 methyl coumarate 37°C, pH 6.0 Talaromyces funiculosus
39.4
-
 methyl ferulate 45°C, pH 6.0, isoenzyme FAE-II Fusarium oxysporum
66
-
 methyl sinapate 45°C, pH 6.0, isoenzyme FAE-II Fusarium oxysporum
116.6
-
 methyl sinapate 45°C, pH 6.0, isoenzyme FAE-II Fusarium oxysporum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3 7
-
 Aspergillus oryzae
4 7
-
 Acetivibrio thermocellus
5
-
-
 Aspergillus awamori
5
-
-
 Aspergillus niger
5.5
-
-
 Streptomyces olivochromogenes
5.6
-
-
 Penicillium expansum
6
-
-
 Neurospora crassa
6.2
-
 isoenzyme FAE-I Neocallimastix sp.
6.7
-
-
 Piromyces sp. 'equi'
7
-
 isoenzyme FAE-I Fusarium oxysporum
7
-
 isoenzyme FAE-II Fusarium oxysporum
7
-
 isoenzyme FAE-II Neocallimastix sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10 stable Acetivibrio thermocellus
4 11 stable Aspergillus awamori
4.5 6 stable Aspergillus oryzae
5.5 6.8 stable, isoenzyme FAE-I Neocallimastix sp.
6 7.5 stable Neurospora crassa
6.4 7.6 stable, isoenzyme FAE-II Neocallimastix sp.
7 9 stable, isoenzyme FAE-I Fusarium oxysporum
7 9 stable, isoenzyme FAE-II Fusarium oxysporum

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus oryzae
-
-
 3.6
Aspergillus niger
-
-
 3.6
Aspergillus awamori
-
-
 3.8
Neocallimastix sp. isoenzyme FAE-I
-
 4.2
Neocallimastix sp. isoenzyme FAE-II
-
 5.7
Acetivibrio thermocellus
-
-
 5.8
Fusarium oxysporum isoenzyme FAE-I
-
 9.5
Fusarium oxysporum isoenzyme FAE-II
-
 9.9