Cloned (Comment) | Organism |
---|---|
gene ace, DNA and amino acid sequence determination and analysis, no polyadenylation signal (AATAAA) was found within the 3'-UTR, although a 14-bp span of poly (A) nucleotides is detected at its end, sequence comparisons, subcloning in Escherichia coli strain JM109, recombinant overexpression of enzyme AChE2 in Pichia pastoris strain GS115 using the methanol-inducible AOX1 promoter | Plutella xylostella |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methamidophos | - |
Plutella xylostella | |
additional information | the recombinant enzyme is sensitive to inhibition by methamidophos and pirimicarb, the calculated IC50 values of the two pesticides are 0.357 and 0.888 mg/l, respectively, and the calculated IC70 values are 0.521 and 0.839 mg/l, respectively | Plutella xylostella | |
pirimicarb | - |
Plutella xylostella |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | Plutella xylostella | - |
choline + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plutella xylostella | Q8MZL2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme AChE2 12.1fold from Pichia pastoris strain GS115 by ammonium sulfate fractionation, procainamide affinity chromatography | Plutella xylostella |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
448.3 | - |
purified recombinant enzyme, pH and temperature not specified in the publication | Plutella xylostella |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | - |
Plutella xylostella | choline + acetate | - |
? | |
acetylthiocholine + H2O | - |
Plutella xylostella | thiocholine + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67000, recombinant enzyme, SDS-PAGE | Plutella xylostella |
Synonyms | Comment | Organism |
---|---|---|
ACE | - |
Plutella xylostella |
acetylcholinesterase 2 | - |
Plutella xylostella |
AChE2 | - |
Plutella xylostella |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Plutella xylostella |