Cloned (Comment) | Organism |
---|---|
gene ACHE, recombinant expression in HEK-293 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,6-dichlorophenolindophenol | causes substrate inhibition at concentrations above 0.25 mM | Homo sapiens | |
acetylthiocholine | substrate inhibition at high concentrations | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
2,6-dichlorophenolindophenol | recombinant enzyme, pH and temperature not specified in the publication | Homo sapiens | |
0.18 | - |
acetylthiocholine | recombinant enzyme, pH and temperature not specified in the publication | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | Homo sapiens | - |
choline + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P22303 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | recombinant enzyme expressed in HEK-293 cells | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-dichlorophenolindophenol acetate + H2O | 2,6-dichlorophenolindophenol acetate computational molecular docking study using the enzyme's crystal structure, PDB ID 4YE7 | Homo sapiens | 2,6-dichlorophenolindophenol + acetate | - |
? | |
acetylcholine + H2O | - |
Homo sapiens | choline + acetate | - |
? | |
acetylthiocholine + H2O | substrate is acetylthiocholine iodide | Homo sapiens | thiocholine + acetate | - |
? | |
additional information | the acetylcholinesterase (AChE) enzyme activity is detected using the 5,5'-dithio-bis(2-nitrobenzoic acid)-based colorimetric assay with measurement of the absorbance at 412 nm. 2,6-Dichloroindophenol acetate is performed as a chromogenic substrate also suitable for AChE activity examination | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AChE | - |
Homo sapiens |