Cloned (Comment) | Organism |
---|---|
Djche1 and Djche2, sequence comparisons | Dugesia japonica |
Protein Variants | Comment | Organism |
---|---|---|
additional information | Djche1 and Djche2 knockdown by dsRNA and RNAi | Dugesia japonica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diazinon | - |
Dugesia japonica | |
additional information | inhibition of DjChE activity by exposing animals to either an organophosphorus pesticides (e.g. diazinon) or carbamates (e.g. physostigmine). At high doses, organophosphorus pesticides are lethal to both insects and humans due to inhibition of AChE and subsequent cholinergic toxicity | Dugesia japonica | |
physostigmine | - |
Dugesia japonica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | Dugesia japonica | - |
choline + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dugesia japonica | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | expression patterns of Djche1 and Djche2 | Dugesia japonica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | - |
Dugesia japonica | choline + acetate | - |
? | |
additional information | the Dugesia japonica enzyme DjChE has intermediate features of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE, EC 3.1.1.8). Two candidate genes, Djche1 and Djche2, are responsible for DjChE activity | Dugesia japonica | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Djche1 | - |
Dugesia japonica |
Djche2 | - |
Dugesia japonica |
More | cf. EC 3.1.1.8 | Dugesia japonica |
planarian cholinesterase | - |
Dugesia japonica |
General Information | Comment | Organism |
---|---|---|
malfunction | DjChE inhibition affects planarian behavior, inhibitors delay the reaction of planarians to heat stress. Simultaneous knockdown of both Djche genes by RNAi similarly results in a delayed heat stress response. Chemical inhibition of DjChE activity increases the worms' ability to adhere to a substrate. But increased substrate adhesion is not observed in Djche1/Djche2 (RNAi) animals or in inhibitor-treated day 11 regenerates, suggesting this phenotype may be modulated by other mechanisms besides ChE inhibition | Dugesia japonica |
additional information | Djche1 and Djche2 enzyme structure homology modelling using Torpedo californica AChE as the template (PDB IDs 2cek and 2w6c, respectively) | Dugesia japonica |