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Literature summary for 3.1.1.7 extracted from

  • Araujo, M.C.; Assis, C.R.D.; Silva, K.C.C.; Souza, K.S.; Azevedo, R.S.; Alves, M.H.M.E.; Silva, L.C.; Silva, V.L.; Adam, M.L.; Carvalho Junior, L.B.; Souza Bezerra, R.; Oliveira, M.B.M.
    Characterization of brain acetylcholinesterase of bentonic fish Hoplosternum littorale perspectives of application in pesticides and metal ions biomonitoring (2018), Aquat. Toxicol., 205, 213-226 .
    View publication on PubMed
Search for more literature for 3.1.1.7

Application

Application Comment Organism
diagnostics the enzyme has potential as biomarker of pesticides and metal ions Hoplosternum littorale

Inhibitors

Inhibitors Comment Organism Structure
Aldicarb
-
 Hoplosternum littorale
As3+
-
 Hoplosternum littorale
BW284c51
-
 Hoplosternum littorale
Carbaryl
-
 Hoplosternum littorale
carbofuran
-
 Hoplosternum littorale
Cd2+
-
 Hoplosternum littorale
chlorpyrifos
-
 Hoplosternum littorale
Cu2+
-
 Hoplosternum littorale
diazinon
-
 Hoplosternum littorale
dichlorvos
-
 Hoplosternum littorale
Diflubenzuron
-
 Hoplosternum littorale
eserine
-
 Hoplosternum littorale
Hg2+
-
 Hoplosternum littorale
iso-ompa
-
 Hoplosternum littorale
neostigmine
-
 Hoplosternum littorale
novaluron
-
 Hoplosternum littorale
parathion-methyl
-
 Hoplosternum littorale
Pb2+
-
 Hoplosternum littorale
pyriproxyfen
-
 Hoplosternum littorale
temephos
-
 Hoplosternum littorale
Zn2+
-
 Hoplosternum littorale

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Hoplosternum littorale
1.47
-
 acetylthiocholine pH 8.0, 30°C Hoplosternum littorale

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no or poor effects by 1 mM of Fe2+, Mn2+, Mg2+, and K+ Hoplosternum littorale

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetylcholine + H2O Hoplosternum littorale
-
 choline + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Hoplosternum littorale
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Hoplosternum littorale
-
central nervous system
-
 Hoplosternum littorale
-
neuron
-
 Hoplosternum littorale
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylcholine + H2O
-
 Hoplosternum littorale choline + acetate
-
 ?
acetylthiocholine + H2O
-
 Hoplosternum littorale thiocholine + acetate
-
 ?
additional information activity detection with chromogenic reagent 5,5'-dithiobis-(2-nitrobenzoic) acid (DTNB) Hoplosternum littorale ?
-
 ?

Synonyms

Synonyms Comment Organism
AChE
-
 Hoplosternum littorale

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Hoplosternum littorale

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 45 over 70% of maximal activity within this range Hoplosternum littorale

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 100% activity remaining Hoplosternum littorale
40
-
 80% activity remaining Hoplosternum littorale
50
-
 no activity remaining Hoplosternum littorale

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8100
-
 acetylthiocholine pH 8.0, 30°C Hoplosternum littorale

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Hoplosternum littorale

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000145
-
 neostigmine pH 8.0, 30°C Hoplosternum littorale
0.0000184
-
 BW284c51 pH 8.0, 30°C Hoplosternum littorale
0.0000247
-
 eserine pH 8.0, 30°C Hoplosternum littorale
0.000035
-
 iso-ompa pH 8.0, 30°C Hoplosternum littorale

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0002
-
 pH 8.0, 30°C Hoplosternum littorale eserine
0.00034
-
 pH 8.0, 30°C Hoplosternum littorale neostigmine
0.00196
-
 pH 8.0, 30°C Hoplosternum littorale BW284c51
0.00483
-
 pH 8.0, 30°C Hoplosternum littorale iso-ompa

General Information

General Information Comment Organism
physiological function acetylcholinesterase (AChE) is a serine hydrolase, whose main function is to modulate neurotransmission at cholinergic synapses. It is, therefore, the primary target of some pesticides and heavy metals. Its inhibition in aquatic organisms has been used as an indicator of the presence of these pollutants in water bodies. Acetylcholinesterase is a key enzyme in the regulation of synaptic transmission. AChE promotes the hydrolysis of the neurotransmitter, acetylcholine, to ensure intermittent nerve impulses (which characterize neuronal communication) while preventing acute cholinergic syndrome that can result if this neurotransmitter accumulates in the nerve endings of the central and peripheral nervous system Hoplosternum littorale

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5510
-
 acetylthiocholine pH 8.0, 30°C Hoplosternum littorale