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Literature summary for 3.1.1.7 extracted from
- Expression and characterization of recombinant Locusta migratoria manilensis acetylcholinesterase 1 in Pichia pastoris (2011), Protein Expr. Purif., 77, 62-67.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris strain KM71 | Locusta migratoria manilensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4,4'-(3-oxo-1,5-pentanediyl)bis(N-allyl-N,N-dimethylanilinium) dibromide | BW284c51 | Locusta migratoria manilensis |  |
| eserine sulfate | - |
Locusta migratoria manilensis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0248 | - |
acetylthiocholine | in 100 mM phosphate buffer, pH 7.4, at 30°C | Locusta migratoria manilensis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Locusta migratoria manilensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and nickel affinity column chromatography. The enzyme is purified 3200fold with a yield of 68% | Locusta migratoria manilensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylthiocholine + H2O | best substrate | Locusta migratoria manilensis | thiocholine + acetate | - |
? | |
| butyrylthiocholine + H2O | worst substrate | Locusta migratoria manilensis | thiocholine + butyrate | - |
? | |
| propionylthiocholine + H2O | - |
Locusta migratoria manilensis | thiocholine + propionate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetylcholinesterase 1 | - |
Locusta migratoria manilensis |
| AChE1 | - |
Locusta migratoria manilensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Locusta migratoria manilensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Locusta migratoria manilensis |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Locusta migratoria manilensis | maximum expression of recombinant AChE1 is achieved after 9 days of induction at 2.5% (v/v) methanol | up |
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