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Literature summary for 3.1.1.7 extracted from
- Recombinant expression and biochemical characterization of the catalytic domain of acetylcholinesterase-1 from the African malaria mosquito, Anopheles gambiae (2009), Insect Biochem. Mol. Biol., 39, 646-653.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the His-tagged Anopheles gambiae AChE1 in Spodoptera frugiperda Sf21 cells using the baculovirus system | Anopheles gambiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetylthiocholine | substrate inhibition | Anopheles gambiae |  |
| BW284c51 | - |
Anopheles gambiae | |
| Carbaryl | - |
Anopheles gambiae | |
| choline | product inhibition | Anopheles gambiae | |
| eserine | - |
Anopheles gambiae | |
| ethopropazine | - |
Anopheles gambiae | |
| malaoxon | - |
Anopheles gambiae | |
| paraoxon | - |
Anopheles gambiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Anopheles gambiae | |
| 0.0639 | - |
Acetylcholine | pH 7.5, 22°C, recombinant enzyme | Anopheles gambiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62921 | - |
2 * 65000, recombinant AChE1, SDS-PAGE, 2 * 62921, AChE1, sequence calculation | Anopheles gambiae |
| 65000 | - |
2 * 65000, recombinant AChE1, SDS-PAGE, 2 * 62921, AChE1, sequence calculation | Anopheles gambiae |
| 130000 | - |
recombinant AChE1, native PAGE and gel filtration | Anopheles gambiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylcholine + H2O | Anopheles gambiae | - |
acetate + choline | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anopheles gambiae | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | AChE1 is N-glycosylated | Anopheles gambiae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged Anopheles gambiae AChE1 and catalytic domain 2500fold from insect cells to near homogeneity by ion exchange chromatography, and lectin affinity, and nickel affinity chromatography | Anopheles gambiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-(beta-methyl)thiocholine iodide + H2O | - |
Anopheles gambiae | acetate + methylthiocholine iodide | - |
? | |
| acetylcholine + H2O | - |
Anopheles gambiae | acetate + choline | - |
? | |
| acetylthiocholine iodide + H2O | - |
Anopheles gambiae | acetate + thiocholine iodide | - |
? | |
| additional information | AChE1 is also activity with propionylthiocholine iodide and butyrylthiocholine iodide | Anopheles gambiae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 65000, recombinant AChE1, SDS-PAGE, 2 * 62921, AChE1, sequence calculation | Anopheles gambiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AChE | - |
Anopheles gambiae |
| AChE1 | - |
Anopheles gambiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Anopheles gambiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Anopheles gambiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.5 | - |
Anopheles gambiae |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | recombinant AChE catalytic domain inhibition kinetic constants, overview | Anopheles gambiae |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Anopheles gambiae | sequence calculation, AChE1 | - |
5.97 |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0659 | - |
- |
Anopheles gambiae | ethopropazine | |
| 0.083 | - |
- |
Anopheles gambiae | eserine | |
| 0.725 | - |
- |
Anopheles gambiae | Carbaryl | |
| 0.836 | - |
- |
Anopheles gambiae | paraoxon | |
| 1.99 | - |
- |
Anopheles gambiae | BW284c51 | |
| 3.28 | - |
- |
Anopheles gambiae | malaoxon | |
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