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Literature summary for 3.1.1.7 extracted from
- Probing the active center gorge of acetylcholinesterase by fluorophores linked to substituted cysteines (2000), J. Biol. Chem., 275, 22401-22408.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A262C | mutant retains hydrolytic activity | Mus musculus |
| H287C | mutant retains hydrolytic activity | Mus musculus |
| Y124C | mutant retains hydrolytic activity | Mus musculus |
| Y124C-acrylodan | mutant retains hydrolytic activity | Mus musculus |
| Y124C-benzyl | mutant retains hydrolytic activity | Mus musculus |
| Y124C-N(CH3)3+ | mutant with increased Km | Mus musculus |
| Y124C-NH3+ | mutant with increased Km | Mus musculus |
| Y124C-SO3- | mutant retains hydrolytic activity | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fasciculin | inhibits catalysis peripherally by sealing the mouth of the active center gorge | Mus musculus | |
| trifluoroacetophenone | inhibits Y124C mutant slower than the wild-type enzyme | Mus musculus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.046 | - |
Acetylcholine | wild-type | Mus musculus | |
| 0.058 | - |
Acetylcholine | H287C mutant | Mus musculus | |
| 0.058 | - |
Acetylcholine | Y124C-SO3- mutant | Mus musculus | |
| 0.059 | - |
Acetylcholine | A262C mutant | Mus musculus | |
| 0.059 | - |
Acetylcholine | Y124C-benzyl mutant | Mus musculus | |
| 0.065 | - |
Acetylcholine | Y124C mutant | Mus musculus | |
| 0.28 | - |
Acetylcholine | Y124C-acrylodan mutant | Mus musculus | |
| 1.13 | - |
Acetylcholine | Y124C-N(Ch3)3+ mutant | Mus musculus | |
| 2.36 | - |
Acetylcholine | Y124C-NH3+ mutant | Mus musculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylcholine + H2O | Mus musculus | - |
choline + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylcholine + H2O | - |
Mus musculus | choline + acetate | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 95 | - |
Acetylcholine | Y124C-acrylodan mutant | Mus musculus | |
| 1330 | - |
Acetylcholine | Y124C-benzyl mutant | Mus musculus | |
| 1333 | - |
Acetylcholine | Y124C-benzyl mutant | Mus musculus | |
| 1850 | - |
Acetylcholine | Y124C-N(Ch3)3+ mutant | Mus musculus | |
| 2130 | - |
Acetylcholine | Y124C-SO3- mutant | Mus musculus | |
| 2133 | - |
Acetylcholine | Y124C-SO3- mutant | Mus musculus | |
| 2330 | - |
Acetylcholine | wild-type | Mus musculus | |
| 2333 | - |
Acetylcholine | wild-type | Mus musculus | |
| 2367 | - |
Acetylcholine | Y124C mutant | Mus musculus | |
| 2370 | - |
Acetylcholine | Y124C mutant | Mus musculus | |
| 2730 | - |
Acetylcholine | A262C mutant | Mus musculus | |
| 2733 | - |
Acetylcholine | A262C mutant | Mus musculus | |
| 3000 | - |
Acetylcholine | H287C mutant | Mus musculus | |
| 4350 | - |
Acetylcholine | Y124C-NH3+ mutant | Mus musculus | |
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Release 2023.1 (January 2023)
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