Cloned (Comment) | Organism |
---|---|
truncated at position 548 | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
F295A/Y337A | mutant retains hydrolytic activity | Mus musculus |
F295L/Y337A | mutant retains hydrolytic activity | Mus musculus |
F297I/Y337A | increased inhibition by RP-cycloheptyl methylphosphonyl thiocholine | Mus musculus |
Y337A/E202Q/F295A | mutant retains hydrolytic activity | Mus musculus |
Y337A/F295A/F297A | 4fold increased Km | Mus musculus |
Y337A/F295L/F297I | mutant retains hydrolytic activity | Mus musculus |
Y337A/F295L/Y338A | no substrate inactivation | Mus musculus |
Y337A/F338A | mutant retains hydrolytic activity | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,3-dimethylbutyl methylphosphonyl thiocholine | - |
Mus musculus | |
Acetylcholine | substrate inhibition | Mus musculus | |
cycloheptyl methylphosphonyl thiocholine | SP and RP enantiomers | Mus musculus | |
isopropyl methylphosphonyl thiocholine | SP and RP enantiomers | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.047 | - |
Acetylcholine | wild-type, pH 7, 22°C | Mus musculus | |
0.069 | - |
Acetylcholine | Y337A/F295L/F297I mutant, pH 7, 22°C | Mus musculus | |
0.076 | - |
Acetylcholine | F295A/Y337A mutant, pH 7, 22°C | Mus musculus | |
0.1 | - |
Acetylcholine | F295L/Y337A mutant, pH 7, 22°C | Mus musculus | |
0.1 | - |
Acetylcholine | Y337A/E202Q/F295A mutant, pH 7, 22°C | Mus musculus | |
0.15 | - |
Acetylcholine | Y337A/F295L/Y338A mutant, pH 7, 22°C | Mus musculus | |
0.15 | - |
Acetylcholine | Y337A/F338A mutant, pH 7, 22°C | Mus musculus | |
0.19 | - |
Acetylcholine | Y337A/F295A/F297A mutant, pH 7, 22°C | Mus musculus | |
0.34 | - |
Acetylcholine | F297I/Y337A mutant, pH 7, 22°C | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P21836 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | - |
Mus musculus | choline + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | truncated enzyme | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
Acetylcholine | F295A/Y337A mutant, pH 7, 22°C | Mus musculus | |
0.7 | - |
Acetylcholine | F295L/Y337A mutant, pH 7, 22°C | Mus musculus | |
0.97 | - |
Acetylcholine | Y337A/E202Q/F295A mutant, pH 7, 22°C | Mus musculus | |
16.9 | - |
Acetylcholine | F297I/Y337A mutant, pH 7, 22°C | Mus musculus | |
18.3 | - |
Acetylcholine | Y337A/F295A/F297A mutant, pH 7, 22°C | Mus musculus | |
23.3 | - |
Acetylcholine | F297I/Y337A mutant, pH 7, 22°C | Mus musculus | |
25 | - |
Acetylcholine | Y337A/F295L/F297I mutant, pH 7, 22°C | Mus musculus | |
33.33 | - |
Acetylcholine | F295A/Y337A mutant, pH 7, 22°C | Mus musculus | |
65 | - |
Acetylcholine | Y337A/F338A mutant, pH 7, 22°C | Mus musculus | |
66.67 | - |
Acetylcholine | F295L/Y337A mutant, pH 7, 22°C | Mus musculus | |
250 | - |
Acetylcholine | wild-type, pH 7, 22°C | Mus musculus |