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Literature summary for 3.1.1.67 extracted from

  • Kaphalia, B.S.; Ansari, G.A.S.
    Purification and characterization of rat hepatic microsomal low molecular weight fatty acid ethyl ester synthase and its relationship to carboxylesterases (2001), J. Biochem. Mol. Toxicol., 15, 165-171.
    View publication on PubMed
Search for more literature for 3.1.1.67

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome 180 kDa high molecular weight major protein fraction and 60 kDa low molecular weight minor protein fraction in liver microsomes with FAEES activity Rattus norvegicus
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 low molecular weight minor protein fraction in liver microsomes with FAEES activity, gel filtration Rattus norvegicus
180000
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 high molecular weight major protein fraction in liver microsomes with FAEES activity, gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
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-
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Purification (Commentary)

Purification (Comment) Organism
5 distinct 60 kDa low molecular weight proteins, 10.2-32.5fold Rattus norvegicus
from liver microsomes, 180 kDa high molecular weight major protein fraction and 60 kDa low molecular weight minor protein fraction Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver 180 kDa high molecular weight major protein fraction and 60 kDa low molecular weight minor protein fraction in liver microsomes with FAEES activity Rattus norvegicus
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0111
-
 60 kDa low molecular weight protein from liver microsomes with pI 5.3 Rattus norvegicus
0.0115
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 60 kDa low molecular weight protein from liver microsomes with pI 6.5 Rattus norvegicus
0.0125
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 60 kDa low molecular weight protein from liver microsomes with pI 5.0 Rattus norvegicus
0.0133
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 60 kDa low molecular weight protein from liver microsomes with pI 5.6 Rattus norvegicus
0.035
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 60 kDa low molecular weight protein from liver microsomes with pI 5.8 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fatty acid + ethanol
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 Rattus norvegicus fatty acyl ethyl ester + H2O
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 ?
additional information structural and functional relationship to hepatic microsomal carboxylesterases, low molecular weight FAEE synthase activity most probably is expressed by various isozymes of hepatic microsomal carboxylesterases, which are also involved in the biotransformation of xenobiotic alcohols and amines, the LMW protein fraction also exhibits fatty acid 2-chloroethyl ester synthase and p-nitrophenyl acetate-hydrolyzing activity Rattus norvegicus ?
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 ?

Subunits

Subunits Comment Organism
monomer 1 * 60000, low molecular weight minor protein fraction, SDS-PAGE Rattus norvegicus
trimer 3 * 60000, high molecular weight major protein fraction, SDS-PAGE Rattus norvegicus

pI Value

Organism Comment pI Value Maximum pI Value
Rattus norvegicus one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing
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 5
Rattus norvegicus one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing
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 5.3
Rattus norvegicus one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing 5.9 5.6
Rattus norvegicus one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing
-
 5.8
Rattus norvegicus one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing
-
 6.5