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Literature summary for 3.1.1.67 extracted from
- Fatty acid ethyl ester synthase in rat adipose tissue and its relationship to carboxylesterase (1992), J. Biol. Chem., 267, 23489-23494.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diisopropylfluorophosphate | 0.001 mM, 50% inhibition after 10 min preincubation at 37°C | Rattus norvegicus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.71 | - |
ethanol | cosubstrate oleate | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 60000, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fatty acid + ethanol | Rattus norvegicus | enzyme contributes to the nonoxidative ethanol metabolism in various organs | fatty acyl ethyl ester + H2O | - |
? | |
| fatty acid + ethanol | Rattus norvegicus | production of a nonoxidative metabolite of ethanol that accumulates in vivo with potentially toxic effects | fatty acyl ethyl ester + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
Wistar rats weighing 300-400 g | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from adipose tissue, 2 enzyme forms | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipose tissue | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| lung | - |
Rattus norvegicus | - |
| additional information | not: kidney | Rattus norvegicus | - |
| testis | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
| 0.015 | - |
pH 7, 37°C | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fatty acid + ethanol | substrate specificity | Rattus norvegicus | fatty acyl ethyl ester + H2O | - |
? | |
| fatty acid + ethanol | enzyme contributes to the nonoxidative ethanol metabolism in various organs | Rattus norvegicus | fatty acyl ethyl ester + H2O | - |
? | |
| fatty acid + ethanol | production of a nonoxidative metabolite of ethanol that accumulates in vivo with potentially toxic effects | Rattus norvegicus | fatty acyl ethyl ester + H2O | - |
? | |
| monoacylglycerol + H2O | hydrolysis, susceptibilities of substrates increase with decreasing acyl chain length of the fatty acid moiety | Rattus norvegicus | fatty acid + glycerol | - |
? | |
| additional information | FAEES is associated with carboxylesterase in adipose tissue | Rattus norvegicus | ? | - |
? | |
| oleate + ethanol | ethyl oleate-hydrolyzing activity is about one-eighth of the synthesizing activity | Rattus norvegicus | ethyl oleate + H2O | - |
r | |
| triacylglycerol + H2O | hydrolysis, susceptibilities of substrates increase with decreasing acyl chain length of the fatty acid moiety | Rattus norvegicus | ? | - |
? | |
| triolein + H2O | - |
Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
optimum and assay at | Rattus norvegicus |
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Release 2023.1 (January 2023)
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