BRENDA - Enzyme Database show
show all sequences of 3.1.1.64

Identification of the key residues determining the product specificity of isomerohydrolase

Takahashi, Y.; Moiseyev, G.; Nikolaeva, O.; Ma, J.X.; Biochemistry 51, 4217-4225 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of wild-type enzyme and mutants in HEK-293A cells
Danio rerio
recombinant expression in HEK-293A cells
Gallus gallus
Engineering
Amino acid exchange
Commentary
Organism
F103L
site-directed mutagenesis, the mutation reverses the enzyme isomerization product specificity from formation of 11-cis-retinol to 13-cis-retinol, product of EC 3.1.1.90. Formation of 95.7% 13-cis-retinol and 4.3% 11-cis-retinol
Danio rerio
K222M
site-directed mutagenesis, the mutation reverses the enzyme isomerization product specificity from formation of 11-cis-retinol to 13-cis-retinol, product of EC 3.1.1.90. Formation of 55.1% 13-cis-retinol and 44.9% 11-cis-retinol
Danio rerio
L133S
site-directed mutagenesis, the mutation reverses the enzyme isomerization product specificity from formation of 11-cis-retinol to 13-cis-retinol, product of EC 3.1.1.90. Formation of 71-3% 13-cis-retinol and 28.7% 11-cis-retinol
Danio rerio
Y58N
site-directed mutagenesis, the mutation completely reverses the enzyme isomerization product specificity from formation of 11-cis-retinol to 13-cis-retinol, product of EC 3.1.1.90. Exclusive formation of 13-cis-retinol
Danio rerio
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
an all-trans-retinyl ester + H2O
Gallus gallus
the enzyme generates predominantly 11-cis-retinol and a minor amount of 13-cis-retinol, from all-trans-retinyl ester
11-cis-retinol + a fatty acid
-
-
?
an all-trans-retinyl ester + H2O
Danio rerio
the enzyme generates predominantly 11-cis-retinol and a minor amount of 13-cis-retinol, from all-trans-retinyl ester
11-cis-retinol + a fatty acid
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Danio rerio
-
homologue RPE65c
-
Gallus gallus
-
homologue RPE65
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
epithelium
-
Danio rerio
-
epithelium
-
Gallus gallus
-
additional information
enzyme homologue RPE65c is expressed in retinal Müller cells, not in the retinal pigment epithelium. Zebrafish is a cone-dominant species with 79% cones and 21% rods
Danio rerio
-
Mueller cell
-
Danio rerio
-
retina
inner
Danio rerio
-
retina
-
Gallus gallus
-
retinal pigment epithelium
-
Gallus gallus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
an all-trans-retinyl ester + H2O
the enzyme generates predominantly 11-cis-retinol and a minor amount of 13-cis-retinol, from all-trans-retinyl ester
729213
Gallus gallus
11-cis-retinol + a fatty acid
-
-
-
?
an all-trans-retinyl ester + H2O
the enzyme generates predominantly 11-cis-retinol and a minor amount of 13-cis-retinol, from all-trans-retinyl ester
729213
Danio rerio
11-cis-retinol + a fatty acid
-
-
-
?
an all-trans-retinyl ester + H2O
the enzyme generates predominantly 11-cis-retinol (72.2%) and a minor amount of 13-cis-retinol (27.8%), from all-trans-retinyl ester
729213
Danio rerio
11-cis-retinol + a fatty acid
-
-
-
?
an all-trans-retinyl ester + H2O
the enzyme generates predominantly 11-cis-retinol (87.3%) and a minor amount of 13-cis-retinol (12.7%), from all-trans-retinyl ester
729213
Gallus gallus
11-cis-retinol + a fatty acid
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of wild-type enzyme and mutants in HEK-293A cells
Danio rerio
recombinant expression in HEK-293A cells
Gallus gallus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
F103L
site-directed mutagenesis, the mutation reverses the enzyme isomerization product specificity from formation of 11-cis-retinol to 13-cis-retinol, product of EC 3.1.1.90. Formation of 95.7% 13-cis-retinol and 4.3% 11-cis-retinol
Danio rerio
K222M
site-directed mutagenesis, the mutation reverses the enzyme isomerization product specificity from formation of 11-cis-retinol to 13-cis-retinol, product of EC 3.1.1.90. Formation of 55.1% 13-cis-retinol and 44.9% 11-cis-retinol
Danio rerio
L133S
site-directed mutagenesis, the mutation reverses the enzyme isomerization product specificity from formation of 11-cis-retinol to 13-cis-retinol, product of EC 3.1.1.90. Formation of 71-3% 13-cis-retinol and 28.7% 11-cis-retinol
Danio rerio
Y58N
site-directed mutagenesis, the mutation completely reverses the enzyme isomerization product specificity from formation of 11-cis-retinol to 13-cis-retinol, product of EC 3.1.1.90. Exclusive formation of 13-cis-retinol
Danio rerio
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
an all-trans-retinyl ester + H2O
Gallus gallus
the enzyme generates predominantly 11-cis-retinol and a minor amount of 13-cis-retinol, from all-trans-retinyl ester
11-cis-retinol + a fatty acid
-
-
?
an all-trans-retinyl ester + H2O
Danio rerio
the enzyme generates predominantly 11-cis-retinol and a minor amount of 13-cis-retinol, from all-trans-retinyl ester
11-cis-retinol + a fatty acid
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
epithelium
-
Danio rerio
-
epithelium
-
Gallus gallus
-
additional information
enzyme homologue RPE65c is expressed in retinal Müller cells, not in the retinal pigment epithelium. Zebrafish is a cone-dominant species with 79% cones and 21% rods
Danio rerio
-
Mueller cell
-
Danio rerio
-
retina
inner
Danio rerio
-
retina
-
Gallus gallus
-
retinal pigment epithelium
-
Gallus gallus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
an all-trans-retinyl ester + H2O
the enzyme generates predominantly 11-cis-retinol and a minor amount of 13-cis-retinol, from all-trans-retinyl ester
729213
Gallus gallus
11-cis-retinol + a fatty acid
-
-
-
?
an all-trans-retinyl ester + H2O
the enzyme generates predominantly 11-cis-retinol and a minor amount of 13-cis-retinol, from all-trans-retinyl ester
729213
Danio rerio
11-cis-retinol + a fatty acid
-
-
-
?
an all-trans-retinyl ester + H2O
the enzyme generates predominantly 11-cis-retinol (72.2%) and a minor amount of 13-cis-retinol (27.8%), from all-trans-retinyl ester
729213
Danio rerio
11-cis-retinol + a fatty acid
-
-
-
?
an all-trans-retinyl ester + H2O
the enzyme generates predominantly 11-cis-retinol (87.3%) and a minor amount of 13-cis-retinol (12.7%), from all-trans-retinyl ester
729213
Gallus gallus
11-cis-retinol + a fatty acid
-
-
-
?
General Information
General Information
Commentary
Organism
evolution
it is likely that the two novel homologues of RPE65 (13cIMH and RPE65c, EC 3.1.1.90 and EC 3.1.1.64, respectively) are generated through gene duplication after the separation of fish RPE65 from the ancestral RPE65, because they exhibit an extremely high level of sequence identity (97%) and are located in the same chromosome, but on a different chromosome from RPE65
Danio rerio
additional information
key residues determining the isomerization product specificity of the enzyme are Tyr58, Phe103, and Leu133
Danio rerio
physiological function
the efficient recycling of the chromophore of visual pigments, 11-cis-retinal, through the retinoid visual cycle is an essential process for maintaining normal vision. RPE65 is the isomerohydrolase in retinal pigment epithelium and generates predominantly 11-cis-retinol and a minor amount of 13-cis-retinol, from all-trans-retinyl ester. Enzyme homologue RPE65c expressed in the inner retina may serve as an alternative isomerohydrolase in the inner retinal visual cycle to meet the high demand for recycling of the chromophore in the cone-dominant retina
Danio rerio
physiological function
the efficient recycling of the chromophore of visual pigments, 11-cis-retinal, through the retinoid visual cycle is an essential process for maintaining normal vision. RPE65 is the isomerohydrolase in retinal pigment epithelium and generates predominantly 11-cis-retinol and a minor amount of 13-cis-retinol, from all-trans-retinyl ester
Gallus gallus
General Information (protein specific)
General Information
Commentary
Organism
evolution
it is likely that the two novel homologues of RPE65 (13cIMH and RPE65c, EC 3.1.1.90 and EC 3.1.1.64, respectively) are generated through gene duplication after the separation of fish RPE65 from the ancestral RPE65, because they exhibit an extremely high level of sequence identity (97%) and are located in the same chromosome, but on a different chromosome from RPE65
Danio rerio
additional information
key residues determining the isomerization product specificity of the enzyme are Tyr58, Phe103, and Leu133
Danio rerio
physiological function
the efficient recycling of the chromophore of visual pigments, 11-cis-retinal, through the retinoid visual cycle is an essential process for maintaining normal vision. RPE65 is the isomerohydrolase in retinal pigment epithelium and generates predominantly 11-cis-retinol and a minor amount of 13-cis-retinol, from all-trans-retinyl ester. Enzyme homologue RPE65c expressed in the inner retina may serve as an alternative isomerohydrolase in the inner retinal visual cycle to meet the high demand for recycling of the chromophore in the cone-dominant retina
Danio rerio
physiological function
the efficient recycling of the chromophore of visual pigments, 11-cis-retinal, through the retinoid visual cycle is an essential process for maintaining normal vision. RPE65 is the isomerohydrolase in retinal pigment epithelium and generates predominantly 11-cis-retinol and a minor amount of 13-cis-retinol, from all-trans-retinyl ester
Gallus gallus
Other publictions for EC 3.1.1.64
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
752236
Kolesnikov
Examining the role of cone-ex ...
Homo sapiens, Mus musculus
Sci. Rep.
8
14201
2018
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
751807
Sheridan
RPE65 and the accumulation of ...
Mus musculus
Photochem. Photobiol.
93
844-848
2017
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
751079
Eroglu
Inhibition of RPE65 retinol i ...
Homo sapiens
J. Biol. Chem.
291
4966-4973
2016
-
-
1
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
730062
Takahashi
Identification of key residues ...
Homo sapiens
J. Biol. Chem.
289
6743-26751
2014
-
-
1
-
25
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
25
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
715720
Amann
Vitamin A metabolism in benign ...
Homo sapiens
J. Cell. Physiol.
227
718-728
2012
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
729213
Takahashi
Identification of the key resi ...
Danio rerio, Gallus gallus
Biochemistry
51
4217-4225
2012
-
-
2
-
4
-
-
-
-
-
-
2
-
3
-
-
-
-
-
7
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
7
-
-
4
-
-
-
-
-
-
-
-
-
-
4
4
-
-
-
730714
Poliakov
Origin and evolution of retino ...
no activity in cephalochordata, no activity in tunicata, Petromyzon marinus
PLoS ONE
7
e49975
2012
-
-
1
-
-
-
-
-
-
-
-
1
-
10
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
714103
Nikolaeva
Binding to lipid membrane indu ...
Gallus gallus
Biochem. J.
436
591-597
2011
-
-
1
-
-
-
-
-
1
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714267
Poliakov
Aromatic lipophilic spin traps ...
Bos taurus
Biochemistry
50
6739-6741
2011
-
-
-
-
-
-
6
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
6
1
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714986
Takahashi
An alternative isomerohydrolas ...
Danio rerio
FEBS J.
278
2913-2926
2011
-
-
1
-
-
-
1
-
2
1
-
-
-
4
-
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
4
2
-
-
-
-
-
-
-
5
-
-
3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
714991
Takahashi
An enzymatic mechanism for gen ...
Danio rerio
FEBS J.
278
973-987
2011
-
-
1
-
-
-
1
1
2
1
1
-
-
3
-
-
1
-
-
1
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
2
1
1
-
-
-
-
1
-
1
-
-
1
1
-
-
-
1
-
-
-
-
-
1
1
-
-
-
715149
Lopes
The Usher 1B protein, MYO7A, i ...
Mus musculus
Hum. Mol. Genet.
20
2560-2570
2011
1
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
704670
Guignard
FATP1 inhibits 11-cis retinol ...
Homo sapiens
J. Biol. Chem.
285
18759-18768
2010
-
-
1
-
-
-
1
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704672
Redmond
RPE65, visual cycle retinol is ...
Homo sapiens
J. Biol. Chem.
285
1919-1927
2010
-
-
-
-
24
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
24
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704711
Golczak
Importance of membrane structu ...
Homo sapiens
J. Biol. Chem.
285
9667-9682
2010
1
-
-
1
-
-
-
-
1
-
-
-
-
2
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
706574
Kiser
Membrane-binding and enzymatic ...
Bos taurus
Prog. Retin. Eye Res.
29
428-442
2010
-
-
-
-
-
-
-
-
2
2
1
-
-
1
-
-
1
-
-
1
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
1
-
-
-
-
1
-
1
-
-
3
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
714020
Nikolaeva
Negative charge of the glutami ...
Homo sapiens
Biochem. Biophys. Res. Commun.
391
1757-1761
2010
-
-
1
-
2
-
-
-
2
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
715577
Yuan
Rpe65 isomerase associates wit ...
Bos taurus, Gallus gallus
J. Biol. Chem.
285
988-999
2010
1
-
1
-
-
-
-
-
2
-
-
-
-
2
-
1
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
716114
Travis
Analysis of the retinoid isome ...
Bos taurus, Gallus gallus
Methods Mol. Biol.
652
329-339
2010
-
-
-
-
-
-
-
-
2
-
-
-
-
3
-
-
-
-
-
6
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
706518
Kiser
Crystal structure of native RP ...
Bos taurus
Proc. Natl. Acad. Sci. USA
106
17325-17330
2009
-
-
-
1
-
-
-
-
1
1
-
-
-
2
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714951
Nikolaeva
Purified RPE65 shows isomerohy ...
Gallus gallus
FEBS J.
276
3020-3030
2009
-
-
1
-
-
1
1
1
1
-
-
-
-
2
-
-
1
-
-
1
-
-
3
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
1
1
-
-
-
-
-
-
1
-
1
-
-
3
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
715501
Takahashi
Identification of a novel palm ...
Bos taurus, Homo sapiens
J. Biol. Chem.
284
3211-3218
2009
2
-
1
-
2
-
-
-
4
-
2
-
-
4
-
-
-
-
-
2
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
2
-
-
-
-
-
4
-
2
-
-
-
-
-
-
2
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698774
Radu
Retinal pigment epithelium-ret ...
Mus musculus
J. Biol. Chem.
283
19730-19738
2008
-
-
-
-
1
-
1
-
-
-
-
-
-
4
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715496
Moiseyev
RPE65 from cone-dominant chick ...
Bos taurus, Gallus gallus, Homo sapiens
J. Biol. Chem.
283
8110-8117
2008
-
-
3
-
-
-
6
-
3
-
1
-
-
6
-
-
1
-
-
3
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
6
-
-
3
-
1
-
-
-
-
1
-
3
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
679833
Chen
Impacts of two point mutations ...
Homo sapiens
FEBS Lett.
580
4200-4204
2006
-
-
-
-
2
1
-
-
2
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
680672
Takahashi
Two point mutations of RPE65 f ...
Homo sapiens
J. Biol. Chem.
281
21820-21826
2006
-
-
1
-
2
1
-
-
2
-
-
-
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
1
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714161
Maiti
Small molecule RPE65 antagonis ...
Bos taurus
Biochemistry
45
852-860
2006
-
-
-
-
-
-
1
-
1
-
-
1
-
2
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
715484
Moiseyev
RPE65 is an iron(II)-dependent ...
Bos taurus
J. Biol. Chem.
281
2835-2840
2006
-
-
1
-
-
-
2
-
1
2
-
-
-
2
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
2
2
-
-
1
2
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
664542
Jin
Rpe65 is the retinoid isomeras ...
Bos taurus
Cell
122
449-459
2005
-
-
1
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714158
Lyubarsky
Mole quantity of RPE65 and its ...
Mus musculus
Biochemistry
44
9880-9888
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
3
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
715009
Takahashi
Identification of conserved hi ...
Homo sapiens
FEBS Lett.
579
5414-5418
2005
-
-
1
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
716734
Redmond
Mutation of key residues of RP ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
102
13658-13663
2005
-
-
1
-
23
-
1
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
23
-
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715473
Mata
Rpe65 is a retinyl ester bindi ...
Mus musculus
J. Biol. Chem.
279
635-643
2004
1
-
1
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714150
Gollapalli
RPE65 operates in the vertebra ...
Bos taurus
Biochemistry
42
11824-11830
2003
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
714151
Moiseyev
Retinyl esters are the substra ...
Bos taurus, Mus musculus
Biochemistry
42
2229-2238
2003
-
-
-
-
-
-
2
-
1
-
-
-
-
5
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715454
McBee
Isomerization of 11-cis-retino ...
Bos taurus
J. Biol. Chem.
276
48483-48493
2001
-
-
-
-
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
170657
Mata
Substrate specificity of retin ...
Bos taurus
J. Lipid Res.
39
604-612
1998
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
716308
Redmond
Rpe65 is necessary for product ...
Mus musculus
Nat. Genet.
20
344-351
1998
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
170660
Mata
Comparison of retinyl ester hy ...
Bos taurus
J. Lipid Res.
37
1947-1952
1996
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
170654
Bustamante
Retinyl ester hydrolase and th ...
Gallus gallus
Am. J. Physiol.
269
R1346-R1350
1995
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
170636
Cooper
Hydrolysis of cis and trans is ...
Sus scrofa
Arch. Biochem. Biophys.
260
705-711
1988
-
-
-
-
-
-
1
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
170637
Blaner
Hydrolysis of 11-cis- and all- ...
Homo sapiens
J. Biol. Chem.
262
53-58
1987
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
170640
Blaner W.S.; Prystowsky
Rat liver retinyl palmitate hy ...
Bos taurus
Biochim. Biophys. Acta
794
419-427
1984
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-