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Literature summary for 3.1.1.64 extracted from

  • Moiseyev, G.; Takahashi, Y.; Chen, Y.; Gentleman, S.; Redmond, T.M.; Crouch, R.K.; Ma, J.X.
    RPE65 is an iron(II)-dependent isomerohydrolase in the retinoid visual cycle (2006), J. Biol. Chem., 281, 2835-2840.
    View publication on PubMed
Search for more literature for 3.1.1.64

Cloned(Commentary)

Cloned (Comment) Organism
expressed in 293A-LRAT cells Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline dose-dependent inhibition Bos taurus
2,2'-bipyridine dose-dependent inhibition Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Bos taurus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ Fe2+ is essential for the isomerohydrolase activity Bos taurus
additional information not affected by Fe(III) citrate, Fe3+, Cu2+, Zn2+, or Mg2+ Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
retinal pigment epithelium
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
all-trans-retinyl ester + H2O
-
 Bos taurus 11-cis-retinol + a fatty acid
-
 ?

Synonyms

Synonyms Comment Organism
Rpe65
-
 Bos taurus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.2
-
 in 10 mM 1,3-bis[tris(hydroxymethyl)methylamino]propane, pH 8.0, 100 mM NaCl, at 37°C Bos taurus 1,10-phenanthroline
0.5
-
 in 10 mM 1,3-bis[tris(hydroxymethyl)methylamino]propane, pH 8.0, 100 mM NaCl, at 37°C Bos taurus 2,2'-bipyridine