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Literature summary for 3.1.1.64 extracted from

  • Takahashi, Y.; Moiseyev, G.; Chen, Y.; Ma, J.X.
    Identification of conserved histidines and glutamic acid as key residues for isomerohydrolase activity of RPE65, an enzyme of the visual cycle in the retinal pigment epithelium (2005), FEBS Lett., 579, 5414-5418.
    View publication on PubMed
Search for more literature for 3.1.1.64

Cloned(Commentary)

Cloned (Comment) Organism
expressed in 293A-LRAT cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
H180A the mutant shows about 20% decreased protein levels and abolished enzymatic activity Homo sapiens
H241A the mutant shows about 80% decreased protein levels and abolished enzymatic activity and significantly decreased protein stabilities in the presence of cycloheximide Homo sapiens
H313A the mutant shows about 10% decreased protein levels and abolished enzymatic activity and significantly decreased protein stabilities in the presence of cycloheximide Homo sapiens
H527A the mutant shows about 20% decreased protein levels and abolished enzymatic activity and significantly decreased protein stabilities in the presence of cycloheximide Homo sapiens

General Stability

General Stability Organism
wild type RPE65 has a half-life of more than 10 h after the addition of cycloheximide Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
retinal pigment epithelium
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
all-trans-retinyl ester + H2O
-
 Homo sapiens 11-cis-retinol + a fatty acid
-
 ?

Synonyms

Synonyms Comment Organism
Rpe65
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function RPE65 from the retinal pigment epithelium is a critical enzyme in the visual cycle for regeneration of 11-cis retinal Homo sapiens