Cloned (Comment) | Organism |
---|---|
expressed in 293A-LRAT cells | Gallus gallus |
General Stability | Organism |
---|---|
0.1% (w/v) CHAPS is optimal for solubilizing RPE65 while preserving its enzymatic activity | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CHAPS | the addition of 0.5% (w/v) CHAPS to the assay system almost completely abolishes the 11-cis-retinol formation | Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
all-trans-retinyl palmitate | purified enzyme, in 10 mM BTP buffer, pH 8.0, 100 mM NaCl, at 37°C | Gallus gallus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Gallus gallus | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
retinal pigment epithelium | - |
Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
all-trans-retinyl ester + H2O | all-trans-retinyl ester must be incorporated into the phospholipid membrane to serve as a substrate for isomerohydrolase RPE65 | Gallus gallus | 11-cis-retinol + a fatty acid | - |
? | |
all-trans-retinyl palmitate + H2O | high affinity substrate | Gallus gallus | 11-cis-retinol + palmitate | - |
? | |
additional information | neither retinyl ester nor 11-cis-retinol is produced after incubation of all-trans-retinol with the purified RPE65 (not an intrinsic substrate for RPE65) | Gallus gallus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Rpe65 | - |
Gallus gallus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000145 | - |
all-trans-retinyl palmitate | purified enzyme, in 10 mM BTP buffer, pH 8.0, 100 mM NaCl, at 37°C | Gallus gallus |
General Information | Comment | Organism |
---|---|---|
physiological function | RPE65 is the isomerohydrolase of the visual cycle | Gallus gallus |