BRENDA - Enzyme Database show
show all sequences of 3.1.1.64

Mole quantity of RPE65 and its productivity in the generation of 11-cis-retinal from retinyl esters in the living mouse eye

Lyubarsky, A.L.; Savchenko, A.B.; Morocco, S.B.; Daniele, L.L.; Redmond, T.M.; Pugh, E.N.; Biochemistry 44, 9880-9888 (2005)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
eye
-
Mus musculus
-
retinal pigment epithelium
-
Mus musculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
all-trans-retinyl ester + H2O
-
714158
Mus musculus
11-cis-retinal + a fatty acid
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * about 65000, SDS-PAGE
Mus musculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
eye
-
Mus musculus
-
retinal pigment epithelium
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
all-trans-retinyl ester + H2O
-
714158
Mus musculus
11-cis-retinal + a fatty acid
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * about 65000, SDS-PAGE
Mus musculus
General Information
General Information
Commentary
Organism
physiological function
the enzyme is essential for the synthesis by isomerohydrolase of 11-cis-retinal, the chromophore of rod and cone opsins
Mus musculus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme is essential for the synthesis by isomerohydrolase of 11-cis-retinal, the chromophore of rod and cone opsins
Mus musculus
Other publictions for EC 3.1.1.64
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
752236
Kolesnikov
Examining the role of cone-ex ...
Homo sapiens, Mus musculus
Sci. Rep.
8
14201
2018
-
-
-
-
-
-
-
-
-
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5
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3
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3
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2
2
-
-
-
751807
Sheridan
RPE65 and the accumulation of ...
Mus musculus
Photochem. Photobiol.
93
844-848
2017
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3
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1
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1
1
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751079
Eroglu
Inhibition of RPE65 retinol i ...
Homo sapiens
J. Biol. Chem.
291
4966-4973
2016
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1
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1
1
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730062
Takahashi
Identification of key residues ...
Homo sapiens
J. Biol. Chem.
289
6743-26751
2014
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1
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25
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1
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4
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1
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1
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25
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1
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-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
715720
Amann
Vitamin A metabolism in benign ...
Homo sapiens
J. Cell. Physiol.
227
718-728
2012
-
-
-
-
-
-
-
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-
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1
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20
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20
-
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-
1
1
-
-
-
729213
Takahashi
Identification of the key resi ...
Danio rerio, Gallus gallus
Biochemistry
51
4217-4225
2012
-
-
2
-
4
-
-
-
-
-
-
2
-
3
-
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7
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4
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2
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4
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2
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7
-
-
4
-
-
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-
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-
-
-
4
4
-
-
-
730714
Poliakov
Origin and evolution of retino ...
no activity in cephalochordata, no activity in tunicata, Petromyzon marinus
PLoS ONE
7
e49975
2012
-
-
1
-
-
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-
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1
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10
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3
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2
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1
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1
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3
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2
-
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-
-
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1
1
-
-
-
714103
Nikolaeva
Binding to lipid membrane indu ...
Gallus gallus
Biochem. J.
436
591-597
2011
-
-
1
-
-
-
-
-
1
-
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2
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1
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1
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1
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1
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1
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1
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-
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-
714267
Poliakov
Aromatic lipophilic spin traps ...
Bos taurus
Biochemistry
50
6739-6741
2011
-
-
-
-
-
-
6
-
1
-
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1
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-
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1
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-
1
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-
-
-
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1
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-
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-
-
-
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-
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6
1
-
1
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-
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-
-
-
1
-
-
1
-
-
-
-
-
-
-
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-
714986
Takahashi
An alternative isomerohydrolas ...
Danio rerio
FEBS J.
278
2913-2926
2011
-
-
1
-
-
-
1
-
2
1
-
-
-
4
-
-
-
-
-
3
-
-
3
-
-
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-
-
-
-
-
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2
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-
1
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4
2
-
-
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-
-
-
-
5
-
-
3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
714991
Takahashi
An enzymatic mechanism for gen ...
Danio rerio
FEBS J.
278
973-987
2011
-
-
1
-
-
-
1
1
2
1
1
-
-
3
-
-
1
-
-
1
-
-
1
1
-
-
-
1
-
-
-
-
-
-
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-
-
1
-
-
-
-
-
1
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1
2
1
1
-
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1
-
1
-
-
1
1
-
-
-
1
-
-
-
-
-
1
1
-
-
-
715149
Lopes
The Usher 1B protein, MYO7A, i ...
Mus musculus
Hum. Mol. Genet.
20
2560-2570
2011
1
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-
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1
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2
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1
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1
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1
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2
-
-
2
-
-
704670
Guignard
FATP1 inhibits 11-cis retinol ...
Homo sapiens
J. Biol. Chem.
285
18759-18768
2010
-
-
1
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-
-
1
-
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-
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-
4
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1
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1
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1
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1
-
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-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
704672
Redmond
RPE65, visual cycle retinol is ...
Homo sapiens
J. Biol. Chem.
285
1919-1927
2010
-
-
-
-
24
-
-
-
-
-
-
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-
1
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2
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24
-
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-
2
-
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-
-
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704711
Golczak
Importance of membrane structu ...
Homo sapiens
J. Biol. Chem.
285
9667-9682
2010
1
-
-
1
-
-
-
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1
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2
-
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1
-
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1
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1
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1
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-
1
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1
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1
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1
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1
-
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-
-
-
-
-
-
-
-
1
1
-
-
-
706574
Kiser
Membrane-binding and enzymatic ...
Bos taurus
Prog. Retin. Eye Res.
29
428-442
2010
-
-
-
-
-
-
-
-
2
2
1
-
-
1
-
-
1
-
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1
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3
1
-
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2
2
1
-
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-
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1
-
1
-
-
3
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
714020
Nikolaeva
Negative charge of the glutami ...
Homo sapiens
Biochem. Biophys. Res. Commun.
391
1757-1761
2010
-
-
1
-
2
-
-
-
2
-
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2
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1
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2
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1
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2
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2
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1
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2
-
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1
1
-
-
-
715577
Yuan
Rpe65 isomerase associates wit ...
Bos taurus, Gallus gallus
J. Biol. Chem.
285
988-999
2010
1
-
1
-
-
-
-
-
2
-
-
-
-
2
-
1
-
-
-
2
-
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3
-
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1
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1
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2
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1
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2
-
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3
-
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716114
Travis
Analysis of the retinoid isome ...
Bos taurus, Gallus gallus
Methods Mol. Biol.
652
329-339
2010
-
-
-
-
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2
-
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3
-
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6
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6
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2
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6
-
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6
-
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706518
Kiser
Crystal structure of native RP ...
Bos taurus
Proc. Natl. Acad. Sci. USA
106
17325-17330
2009
-
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1
-
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1
1
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2
-
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1
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1
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1
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1
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1
1
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1
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1
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1
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714951
Nikolaeva
Purified RPE65 shows isomerohy ...
Gallus gallus
FEBS J.
276
3020-3030
2009
-
-
1
-
-
1
1
1
1
-
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2
-
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1
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1
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3
-
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1
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1
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1
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1
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1
1
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1
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1
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3
-
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1
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1
1
-
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-
715501
Takahashi
Identification of a novel palm ...
Bos taurus, Homo sapiens
J. Biol. Chem.
284
3211-3218
2009
2
-
1
-
2
-
-
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4
-
2
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4
-
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-
-
-
2
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2
2
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2
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1
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2
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4
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2
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2
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2
2
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-
698774
Radu
Retinal pigment epithelium-ret ...
Mus musculus
J. Biol. Chem.
283
19730-19738
2008
-
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1
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1
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4
-
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1
1
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1
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1
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1
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1
1
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1
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-
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-
-
-
-
-
-
-
-
715496
Moiseyev
RPE65 from cone-dominant chick ...
Bos taurus, Gallus gallus, Homo sapiens
J. Biol. Chem.
283
8110-8117
2008
-
-
3
-
-
-
6
-
3
-
1
-
-
6
-
-
1
-
-
3
-
-
3
1
-
-
-
-
-
-
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-
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3
-
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6
-
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3
-
1
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1
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3
-
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3
1
-
-
-
-
-
-
-
-
-
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-
-
-
-
679833
Chen
Impacts of two point mutations ...
Homo sapiens
FEBS Lett.
580
4200-4204
2006
-
-
-
-
2
1
-
-
2
-
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2
-
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1
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2
1
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2
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1
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-
-
-
-
-
-
-
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-
-
1
1
-
-
-
680672
Takahashi
Two point mutations of RPE65 f ...
Homo sapiens
J. Biol. Chem.
281
21820-21826
2006
-
-
1
-
2
1
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714161
Maiti
Small molecule RPE65 antagonis ...
Bos taurus
Biochemistry
45
852-860
2006
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715484
Moiseyev
RPE65 is an iron(II)-dependent ...
Bos taurus
J. Biol. Chem.
281
2835-2840
2006
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664542
Jin
Rpe65 is the retinoid isomeras ...
Bos taurus
Cell
122
449-459
2005
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714158
Lyubarsky
Mole quantity of RPE65 and its ...
Mus musculus
Biochemistry
44
9880-9888
2005
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715009
Takahashi
Identification of conserved hi ...
Homo sapiens
FEBS Lett.
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5414-5418
2005
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716734
Redmond
Mutation of key residues of RP ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
102
13658-13663
2005
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715473
Mata
Rpe65 is a retinyl ester bindi ...
Mus musculus
J. Biol. Chem.
279
635-643
2004
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714150
Gollapalli
RPE65 operates in the vertebra ...
Bos taurus
Biochemistry
42
11824-11830
2003
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714151
Moiseyev
Retinyl esters are the substra ...
Bos taurus, Mus musculus
Biochemistry
42
2229-2238
2003
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715454
McBee
Isomerization of 11-cis-retino ...
Bos taurus
J. Biol. Chem.
276
48483-48493
2001
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170657
Mata
Substrate specificity of retin ...
Bos taurus
J. Lipid Res.
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604-612
1998
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716308
Redmond
Rpe65 is necessary for product ...
Mus musculus
Nat. Genet.
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344-351
1998
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170660
Mata
Comparison of retinyl ester hy ...
Bos taurus
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1947-1952
1996
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Bustamante
Retinyl ester hydrolase and th ...
Gallus gallus
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1995
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Cooper
Hydrolysis of cis and trans is ...
Sus scrofa
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260
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1988
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Blaner
Hydrolysis of 11-cis- and all- ...
Homo sapiens
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1987
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170640
Blaner W.S.; Prystowsky
Rat liver retinyl palmitate hy ...
Bos taurus
Biochim. Biophys. Acta
794
419-427
1984
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