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Literature summary for 3.1.1.64 extracted from
- Membrane-binding and enzymatic properties of RPE65 (2010), Prog. Retin. Eye Res., 29, 428-442.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | the ratio of cytosolic to microsomal RPE65 is approximately 1:2 | Bos taurus | 5829 | - |
| membrane | microsome | Bos taurus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required | Bos taurus |  |
| additional information | Fe3+, Cu2+, Mg2+ and Zn2+ are unable to restore RPE65 isomerase activity | Bos taurus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
x * 65000, SDS-PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified from native bovine RPE microsomes in the presence of octyltetraoxyethylene. Triton X-100 and CHAPS are the most effective agents for extracting RPE65 from microsomal membranes | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| retinal pigment epithelial cell | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| all-trans-retinol | - |
Bos taurus | 11-cis-retinol | - |
? | |
| all-trans-retinyl ester + H2O | - |
Bos taurus | 11-cis-retinol + a fatty acid | - |
? | |
| additional information | all-trans-9-des-methylretinol, retinyl acetate, retinyl heptanoate, all-trans-11-fluororetinol and all-trans-19,19,19-trifluororetinol are not isomerized | Bos taurus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000, SDS-PAGE | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Rpe65 | - |
Bos taurus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | RPE65 is involved not only for regeneration of rhodopsin but also plays an important role in regeneration of cone opsins and is critical for the health of cone photoreceptors. Interactions of RPE65 with an intact phospholipid bilayer are critical for its enzymatic activity | Bos taurus |
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Release 2023.1 (January 2023)
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