Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
Protein Variants | Comment | Organism |
---|---|---|
A137T | 3fold higher enzyme activity than wild-type enzyme in absence of phosphoramidate, in presence of phosphoramidate mutant and wild-type proteins show similar increase in enzyme activity | Salmonella enterica subsp. enterica serovar Typhimurium |
D56N | no activation of enzyme activity in the presence of phosphoramidate | Salmonella enterica subsp. enterica serovar Typhimurium |
H138R | 2.5fold higher enzyme activity than wild-type enzyme in absence of phosphoramidate, in presence of phosphoramidate mutant and wild-type proteins show similar increase in enzyme activity | Salmonella enterica subsp. enterica serovar Typhimurium |
L153P | 2fold higher enzyme activity than wild-type enzyme in absence of phosphoramidate, in presence of phosphoramidate mutant and wild-type proteins show similar increase in enzyme activity | Salmonella enterica subsp. enterica serovar Typhimurium |
R255W | 4fold higher enzyme activity than wild-type enzyme in absence of phosphoramidate, in presence of phosphoramidate mutant and wild-type proteins show similar increase in enzyme activity | Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | phosphorylation of intact enzyme requires Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Salmonella enterica subsp. enterica serovar Typhimurium | demethylation of chemotactic membrane receptors | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation by phosphoramidate requires Mg2+, phosphorylation of the regulatory domain at Asp-56 results in a reorganization of the domain interface, allowing exposure of the active site to the receptor substrate and simultaneously stimulating methylesterase activity | Salmonella enterica subsp. enterica serovar Typhimurium |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
protein L-glutamate O5-methyl ester + H2O = protein L-glutamate + methanol | mechanism of regulation of enzyme activity by the N-terminal domain | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | demethylation of chemotactic membrane receptors | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |