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Literature summary for 3.1.1.60 extracted from

  • Fang, Y.; Zhang, L.; Wang, J.; Zhou, Y.; Ye, B.
    Biodegradation of phthalate esters by a newly isolated Acinetobacter sp. strain LMB-5 and characteristics of its esterase (2017), Pedosphere, 27, 606-615 .
No PubMed abstract available
Search for more literature for 3.1.1.60

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Acinetobacter sp. LMB-5

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 1 mM, 45.5% residual activity Acinetobacter sp. LMB-5
K+ 1 mM, 55.8% residual activity Acinetobacter sp. LMB-5
Mg2+ 1 mM, 15.4% residual activity Acinetobacter sp. LMB-5
Na+ 1 mM, 54.3% residual activity Acinetobacter sp. LMB-5
Zn2+ 1 mM, 21.5% residual activity Acinetobacter sp. LMB-5

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 1 mM, 127% of initial activity Acinetobacter sp. LMB-5
Fe3+ 1 mM, 162% of initial activity Acinetobacter sp. LMB-5

Organic Solvent Stability

Organic Solvent Comment Organism
dimethyl sulfoxide high activity in the presence of 10% v/v Acinetobacter sp. LMB-5
Methanol high activity in the presence of 10% v/v Acinetobacter sp. LMB-5

Organism

Organism UniProt Comment Textmining
Acinetobacter sp. LMB-5
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bis(2-ethylhexyl)phthalate + H2O
-
 Acinetobacter sp. LMB-5 2-ethylhexyl phthalate + 2-ethylhexan-1-ol
-
 ?
dibutylphthalate + H2O
-
 Acinetobacter sp. LMB-5 butylphthalate + butanol
-
 ?
diethylphthalate + H2O
-
 Acinetobacter sp. LMB-5 ethylphthalate + ethanol
-
 ?
dimethylphthalate + H2O
-
 Acinetobacter sp. LMB-5 methylphthalate + methanol
-
 ?

Subunits

Subunits Comment Organism
? x * 40340, calculated from sequence, x * 40000, SDS-PAGE Acinetobacter sp. LMB-5

Synonyms

Synonyms Comment Organism
Est 3563
-
 Acinetobacter sp. LMB-5

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 rapid loss of activity above Acinetobacter sp. LMB-5

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Acinetobacter sp. LMB-5

pH Range

pH Minimum pH Maximum Comment Organism
5 7
-
 Acinetobacter sp. LMB-5

pI Value

Organism Comment pI Value Maximum pI Value
Acinetobacter sp. LMB-5 calculated from sequence
-
 9.1

General Information

General Information Comment Organism
physiological function strains grows in mineral salt medium containing dimethyl phthalate, diethyl phthalate, di-n-butyl phthalate, and di-(2-ethylhexyl) phthalate. The consumption of dimethyl phthalate, diethyl phthalate, di-n-butyl phthalate is up to 98.87%, 94.94%, and 72.15%, respectively Acinetobacter sp. LMB-5