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Literature summary for 3.1.1.60 extracted from
- Achromobacter denitrificans strain SP1 produces an intracellular esterase upon utilizing di(2ethylhexyl)phthalate (2015), Int. Biodeter. Biodegrad., 105, 160-167 .
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | optimum conditions for the production of the maximum (30.5 U) intracellular esterase, are 10 mM di(2-ethylhexyl)phthalate and 72 h incubation at pH 8.0 were found as optimum conditions | Achromobacter denitrificans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.308 | - |
4-nitrophenyl acetate | 50°C, pH 8.0 | Achromobacter denitrificans |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 0.25 mM, maximum esterase activity | Achromobacter denitrificans | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Achromobacter denitrificans | - |
- |
- |
| Achromobacter denitrificans SP1 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 89.5 | - |
substrate 4-nitrophenyl acetate, pH 50°C, pH 8.0 | Achromobacter denitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Achromobacter denitrificans | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Achromobacter denitrificans SP1 | 4-nitrophenol + acetate | - |
? | |
| di(2-ethylhexyl)phthalate + H2O | - |
Achromobacter denitrificans | 2-ethylhexyl phthalate + 2-ethylhexan-1-ol | - |
? | |
| di(2-ethylhexyl)phthalate + H2O | - |
Achromobacter denitrificans SP1 | 2-ethylhexyl phthalate + 2-ethylhexan-1-ol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53000, SDS-PAGE | Achromobacter denitrificans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Achromobacter denitrificans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Achromobacter denitrificans |
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Release 2023.1 (January 2023)
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