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Literature summary for 3.1.1.60 extracted from

  • Krell, H.W.; Sandermann, H.
    Plant biochemistry of xenobiotics. Purification and properties of a wheat esterase hydrolyzing the plasticizer chemical, bis(2-ethylhexyl)phthalate (1984), Eur. J. Biochem., 143, 57-62.
    View publication on PubMed
Search for more literature for 3.1.1.60

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Triticum aestivum

Inhibitors

Inhibitors Comment Organism Structure
4-mercuribenzoate complete inhibition at 1 mM, 80% inhibition at 0.1 mM Triticum aestivum
4-nitrophenyl phosphate
-
 Triticum aestivum
4-nitrophenyl-diethyl phosphate synonym: Paraoxon, complete inhibition at 0.1 and 1 mM Triticum aestivum
cholate
-
 Triticum aestivum
deoxycholate
-
 Triticum aestivum
diisopropylfluorophosphate complete inhibition at 1 mM, 80% inhibition at 0.1 mM Triticum aestivum
Dimethylsulfoxide 70% inihibition at 5% Triticum aestivum
dodecyl beta-maltoside
-
 Triticum aestivum
eserine complete inhibition at 1 mM, 80% inhibition at 0.1 mM Triticum aestivum
ethyleneglycolmonomethyl ether 70% inihibition at 5% Triticum aestivum
HgCl2 complete inhibition at 1 mM, 80% inhibition at 0.1 mM Triticum aestivum
Lubrol Px
-
 Triticum aestivum
phenylmethylsulfonyl fluoride 75% inhibition at 1 mM Triticum aestivum
Span-20
-
 Triticum aestivum
taurocholate
-
 Triticum aestivum
Triton X-100
-
 Triticum aestivum
Tween-20
-
 Triticum aestivum
Tween-80
-
 Triticum aestivum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 bis(2-ethylhexyl)phthalate 30°C Triticum aestivum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 x * 22000, SDS-PAGE Triticum aestivum
38000
-
 gel filtration Triticum aestivum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
bis(2-ethylhexyl)phthalate + H2O Triticum aestivum
-
 2-ethylhexyl phthalate + 2-ethylhexan-1-ol
-
 ?

Organism

Organism UniProt Comment Textmining
Triticum aestivum
-
 wheat
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00077
-
-
 Triticum aestivum

Storage Stability

Storage Stability Organism
4°C, half-life: 1 week for the enzyme from first steps of purification, 1 day for the enzyme from last steps of purification and purified enzyme Triticum aestivum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acylester + H2O with optimum chain-length C6 to C8, cleavage of bis(2-ethylhexyl)phthalate is 10000times slower than cleavage of 4-nitrophenyl octanoate Triticum aestivum 4-nitrophenol + carboxylic acid
-
 ?
bis(2-ethylhexyl)phthalate + H2O
-
 Triticum aestivum 2-ethylhexyl phthalate + 2-ethylhexan-1-ol
-
 ?

Subunits

Subunits Comment Organism
? x * 22000, SDS-PAGE Triticum aestivum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Triticum aestivum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0000833
-
 bis(2-ethylhexyl)phthalate
-
 Triticum aestivum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 9 broad Triticum aestivum