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Literature summary for 3.1.1.59 extracted from

  • Tsubota, T.; Minakuchi, C.; Nakakura, T.; Shinoda, T.; Shiotsuki, T.
    Molecular characterization of a gene encoding juvenile hormone esterase in the red flour beetle, Tribolium castaneum (2010), Insect Mol. Biol., 19, 527-535.
    View publication on PubMed
Search for more literature for 3.1.1.59

Cloned(Commentary)

Cloned (Comment) Organism
genes Tcjhe-a, Tcjhe-b, Tcjhe-c, Tcjhe-d, and Tcjhe-e, encoding isozymes that vary only in the 5'-UTR sequences, DNA and amino acid sequence determination and analysis, intron-exon structure, phylogenetic analysis, overview, recombinant expression of JHE as C-terminal V5-His6-tagged protein in Spodoptera frugiperda Sf9 cells using the baculovirus system Tribolium castaneum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000657
-
 juvenile hormone III pH and temperature not specified in the publication Tribolium castaneum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
juvenile hormone III + H2O Tribolium castaneum degradation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Tribolium castaneum D7US45 genes Tcjhe-a, Tcjhe-b, Tcjhe-c, Tcjhe-d, and Tcjhe-e; genes Tcjhe-a, Tcjhe-b, Tcjhe-c, Tcjhe-d, and Tcjhe-e, encoding isozymes that vary only in the 5'-UTR sequences
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information expression of Tcjhe during development Tribolium castaneum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
juvenile hormone III + H2O
-
 Tribolium castaneum ?
-
 ?
juvenile hormone III + H2O degradation Tribolium castaneum ?
-
 ?
methyl heptylthioacetothioate + H2O the recombinant JHE has a strong ability to degrade methyl hepthylthioacetothioate, HEPTAT, as a synthetic substrate Tribolium castaneum ?
-
 ?

Synonyms

Synonyms Comment Organism
JHE
-
 Tribolium castaneum
Juvenile hormone esterase
-
 Tribolium castaneum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.164
-
 juvenile hormone III pH and temperature not specified in the publication Tribolium castaneum

General Information

General Information Comment Organism
physiological function JHEs are required for the degradation of juvenile hormones in insects Tribolium castaneum