Literature summary for 3.1.1.59 extracted from

Wogulis, M.; Wheelock, C.E.; Kamita, S.G.; Hinton, A.C.; Whetstone, P.A.; Hammock, B.D.; Wilson, D.K.
Structural studies of a potent insect maturation inhibitor bound to the juvenile hormone esterase of Manduca sexta (2006), Biochemistry, 45, 4045-4057.

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Cloned(Commentary)

Cloned (Comment)	Organism
native and mutant enzyme expressed in High 5 cells	Manduca sexta

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop method, 2.7 A crystal structure of the enzyme in complex with the transition state analogue inhibitor 3-octylthio-1,1,1-trifluoropropan-2-one covalently bound to the active site	Manduca sexta
in complex with 3-octylthio-1,1,1-trifluoropropan-2-one, hanging drop vapor diffusion method	Manduca sexta

Protein Variants

Protein Variants	Comment	Organism
F259I	lower affinity for 3-octylthio-1,1,1-trifluoropropan-2-one	Manduca sexta

Inhibitors

Inhibitors	Comment	Organism	Structure
3-Octylthio-1,1,1-trifluoropropan-2-one	transition state analogue inhibitor; transition state analogue inhibitor, IC50 (wild type): 22 nM, IC50 (F259I mutant): 330 nM	Manduca sexta

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
juvenile hormone + H2O	Manduca sexta	key enzyme in the regulation of juvenile hormone level, degradation of juvenile hormone is required for proper insect development	Juvenile hormone acid + methanol	-	?

Organism

Organism	UniProt	Comment	Textmining
Manduca sexta	P19985	-	-
Manduca sexta	Q9GPG0	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Manduca sexta

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
juvenile hormone + H2O	key enzyme in the regulation of juvenile hormone level, degradation of juvenile hormone is required for proper insect development	Manduca sexta	Juvenile hormone acid + methanol	-	?

Synonyms

Synonyms	Comment	Organism
MsJHE	-	Manduca sexta

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.000022	-	transition state analogue inhibitor, IC50 (wild type): 22 nM	Manduca sexta	3-Octylthio-1,1,1-trifluoropropan-2-one
0.00033	-	IC50 (F259I mutant): 330 nM	Manduca sexta	3-Octylthio-1,1,1-trifluoropropan-2-one

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Release 2023.1 (January 2023)