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Literature summary for 3.1.1.59 extracted from
- Juvenile hormone esterase (JHE) from Tenebrio molitor: full-length cDNA sequence, in vitro expression, and characterization of the recombinant protein (2003), Insect Biochem. Mol. Biol., 33, 477-487.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| from fat bodies of pupae, full-length cDNA, expression in Hi-5 cells from Trichoplusia ni, sequencing | Tenebrio molitor |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,1,1-trifluoro-3-(octane-1-sulfonyl)propan-2,2-diol | IC50: 0.0016 mM | Tenebrio molitor |  |
| 1,1,1-trifluoro-3-decylsulfanyl-propan-2-one | potent inhibitor, IC50: 3.6 nm | Tenebrio molitor | |
| 1,1,1-trifluoro-3-octylsulfanyl-propan-2-one | potent inhibitor, IC50: 4.6 nm | Tenebrio molitor | |
| Carbaryl | weak, IC50: above 0.1 mM | Tenebrio molitor | |
| methamidophos | weak, IC50: above 1 mM | Tenebrio molitor | |
| O-ethyl-S-phenyl-phosphoramidothiolate | IC50: 0.0084 mM | Tenebrio molitor | |
| Phenylmethylsulfonylfluoride | mediocre inhibitor, IC50: 0.24 mM | Tenebrio molitor | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000315 | - |
juvenile hormone III | recombinant JHE | Tenebrio molitor | |
| 0.0191 | - |
methyl 1-heptylthioacetothioate | recombinant JHE | Tenebrio molitor |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | recombinant JHE | Tenebrio molitor | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63600 | - |
1 * 66000, recombinant JHE, SDS-PAGE, 1 * 63600, recombinant JHE, sequence calculation | Tenebrio molitor |
| 66000 | - |
1 * 66000, recombinant JHE, SDS-PAGE, 1 * 63600, recombinant JHE, sequence calculation | Tenebrio molitor |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| juvenile hormone + H2O | Tenebrio molitor | selective enzyme, juvenile hormone metabolism, alters the activity of juvenile hormone, which regulates development and reproduction | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tenebrio molitor | Q8WRD8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant JHE, partial, 2.5fold | Tenebrio molitor |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fat body | of 0-48 hours old pupae | Tenebrio molitor | - |
| hemolymph | the cDNA contains an independent secretory signal peptide and is likely to represent a specific isoform that would be present in the hemolymph in vivo | Tenebrio molitor | - |
| pupa | 0-48 hours old, fat bodies | Tenebrio molitor | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.9 | - |
recombinant JHE, hydrolysis of alpha-naphthyl acetate | Tenebrio molitor |
| 1.1 | - |
recombinant JHE, hydrolysis of juvenile hormone III | Tenebrio molitor |
| 5.3 | - |
recombinant JHE, hydrolysis of p-nitrophenyl acetate | Tenebrio molitor |
| 15.1 | - |
recombinant JHE, hydrolysis of HEPTAT | Tenebrio molitor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-naphthyl acetate + H2O | - |
Tenebrio molitor | alpha-naphthol + acetate | - |
? | |
| HEPTAT + H2O | synthetic juvenile hormone analog | Tenebrio molitor | ? | - |
? | |
| juvenile hormone + H2O | selective enzyme, juvenile hormone metabolism, alters the activity of juvenile hormone, which regulates development and reproduction | Tenebrio molitor | ? | - |
? | |
| juvenile hormone III + H2O | hydrolyzes the methyl ester of juvenile hormone, highest specificity for juvenile hormone III | Tenebrio molitor | ? | - |
? | |
| juvenile hormone III + H2O | - |
Tenebrio molitor | juvenile hormone III acid + methanol | - |
? | |
| methyl 1-heptylthioacetothioate + H2O | HEPTAT | Tenebrio molitor | ? | - |
? | |
| p-nitrophenyl acetate + H2O | - |
Tenebrio molitor | p-nitrophenol + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 66000, recombinant JHE, SDS-PAGE, 1 * 63600, recombinant JHE, sequence calculation | Tenebrio molitor |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| JHE | - |
Tenebrio molitor |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
the activity decreases significantly below pH 5 | Tenebrio molitor |
| 5.2 | 10.8 | active at a wide range of pH, at least 80% of the maximum activity in the pH-range | Tenebrio molitor |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Tenebrio molitor | recombinant JHE, isoelectric focusing, pH-gradient: pH 3-7 | - |
4.9 |
| Tenebrio molitor | recombinant JHE, theoretical pI | - |
5.3 |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0000036 | - |
potent inhibitor, IC50: 3.6 nm | Tenebrio molitor | 1,1,1-trifluoro-3-decylsulfanyl-propan-2-one | |
| 0.0000046 | - |
potent inhibitor, IC50: 4.6 nm | Tenebrio molitor | 1,1,1-trifluoro-3-octylsulfanyl-propan-2-one | |
| 0.0016 | - |
IC50: 0.0016 mM | Tenebrio molitor | 1,1,1-trifluoro-3-(octane-1-sulfonyl)propan-2,2-diol | |
| 0.0084 | - |
IC50: 0.0084 mM | Tenebrio molitor | O-ethyl-S-phenyl-phosphoramidothiolate | |
| 0.1 | - |
weak, IC50: above 0.1 mM | Tenebrio molitor | Carbaryl | |
| 0.24 | - |
mediocre inhibitor, IC50: 0.24 mM | Tenebrio molitor | Phenylmethylsulfonylfluoride | |
| 1 | - |
weak, IC50: above 1 mM | Tenebrio molitor | methamidophos | |
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