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Literature summary for 3.1.1.59 extracted from
- Purification and characterization of juvenile hormone esterase from hemolymph of the Colorado potato beetle (1997), Arch. Insect Biochem. Physiol., 35, 261-277.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0014 | - |
juvenile hormone III | pH 8, major enzyme form with pI 5.6 | Leptinotarsa decemlineata |  |
| 0.0022 | - |
juvenile hormone III | pH 8, minor enzyme form with pI 5.5 | Leptinotarsa decemlineata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
2 * 57000, major form with pI 5.6, the subunits are not linked by disulfide bridges, SDS-PAGE | Leptinotarsa decemlineata |
| 120000 | - |
major form with pI 5.6, nondenaturing PAGE | Leptinotarsa decemlineata |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| juvenile hormone + H2O | Leptinotarsa decemlineata | juvenile hormone degradation | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leptinotarsa decemlineata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 147fold, from the fourth larval instar, at day 3 after molt | Leptinotarsa decemlineata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hemolymph | of last larval instar, enzyme occurs mainly in the hemolymph, JHE activities of hemolymph from animals of different developmental stages | Leptinotarsa decemlineata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activities of different developmental stages | Leptinotarsa decemlineata |
| 0.0124 | - |
pH 8, fourth larval instar, at day 9 after molt | Leptinotarsa decemlineata |
| 0.0135 | - |
pH 8, highest specific activity in the fourth larval instar, at day 3 after molt | Leptinotarsa decemlineata |
| 1.841 | - |
pH 8, purified JHE from the fourth larval instar, at day 3 after molt | Leptinotarsa decemlineata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| juvenile hormone + H2O | juvenile hormone degradation | Leptinotarsa decemlineata | ? | - |
? | |
| juvenile hormone III + H2O | racemic | Leptinotarsa decemlineata | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 57000, major form with pI 5.6, the subunits are not linked by disulfide bridges, SDS-PAGE | Leptinotarsa decemlineata |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| JHE | - |
Leptinotarsa decemlineata |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
juvenile hormone III | pH 8, major enzyme form with pI 5.6 | Leptinotarsa decemlineata | |
| 1.9 | - |
juvenile hormone III | pH 8, minor enzyme form with pI 5.5 | Leptinotarsa decemlineata | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Leptinotarsa decemlineata |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Leptinotarsa decemlineata | two enzyme forms, minor form, isoelectric focusing, pH-gradient of 4-6.5 | - |
5.5 |
| Leptinotarsa decemlineata | two enzyme forms, major form, isoelectric focusing, pH-gradient of 4-6.5 | 5.9 | 5.6 |
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