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Literature summary for 3.1.1.57 extracted from

  • Maruyama, K.
    Purification and properties of 2-pyrone-4,6-dicarboxylate hydrolase (1983), J. Biochem., 93, 557-565.
    View publication on PubMed
Search for more literature for 3.1.1.57

General Stability

General Stability Organism
aged enzyme preparation is fully reactivated by preincubation at 24°C for 10 min with an excess amount of dithiothreitol, cysteine or GSH Pseudomonas straminea

Inhibitors

Inhibitors Comment Organism Structure
Co(NO3)2 inhibits hydrolysis reaction by 59.9% Pseudomonas straminea
Cu(NO3)2 inhibits synthesis reaction Pseudomonas straminea
HgCl2
-
 Pseudomonas straminea
Mn(NO3)2 inhibits hydrolysis reaction by 62.1% Pseudomonas straminea
additional information monoiodoacetate had essentially no effect, Mg(NO3)2, Ni(NO3)2, Ca(NO3)2, Cr(NO3)3 and Fe(NO3)3 shows essentially no effect, KCN and various metal chelating reagents including EDTA, tiron, 1,10-phenanthroline, 2,2'-bipyridine, diethyldithiocarbonate and 8-hydroxyquinoline shows no significant effect Pseudomonas straminea
N-ethylmaleimide
-
 Pseudomonas straminea
p-chloromercuribenzoate
-
 Pseudomonas straminea
Zn(NO3)2 inhibits hydrolysis reaction Pseudomonas straminea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
 4-oxalmesaconate pH 6.5, 24°C Pseudomonas straminea
0.087
-
 2-Pyrone-4,6-dicarboxylate pH 8.5, 24°C Pseudomonas straminea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 gel filtration Pseudomonas straminea
33000
-
 1 * 33000, SDS-PAGE Pseudomonas straminea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-pyrone-4,6-dicarboxylate + H2O Pseudomonas straminea highly specific, various other lactones does not serve as substrates 4-carboxy-2-hydroxyhexa-2,4-dienedioate product isomerizes to 4-oxalmesaconate r

Organism

Organism UniProt Comment Textmining
Pseudomonas straminea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas straminea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
247
-
 purification of hydrolase activity Pseudomonas straminea

Storage Stability

Storage Stability Organism
-20°C, 30% of the activity is lost on storage for 1 year Pseudomonas straminea
-20°C, purified enzyme can be stored for at least 2 months without appreciable loss in activity Pseudomonas straminea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-pyrone-4,6-dicarboxylate + H2O highly specific Pseudomonas straminea 4-carboxy-2-hydroxyhexa-2,4-dienedioate product isomerizes to 4-oxalmesaconate r
2-pyrone-4,6-dicarboxylate + H2O highly specific, various other lactones does not serve as substrates Pseudomonas straminea 4-carboxy-2-hydroxyhexa-2,4-dienedioate product isomerizes to 4-oxalmesaconate r
additional information no hydrolysis of gamma-butyrolactone, gamma-valerolactone, epsilon-caprolactone, gamma-n-decalactone, D-glucorono-gamma-lactone, mevalonolactone, 2-pyrone-6-carboxylate, coumalic acid, isodehydracetic acid, 4-hydroxy-6-methyl-2-pyrone and 4-methoxy-6-methyl-2-H-pyran-2-one Pseudomonas straminea ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 33000, SDS-PAGE Pseudomonas straminea

Synonyms

Synonyms Comment Organism
2-pyrone-4,6-dicarboxylate hydrolase
-
 Pseudomonas straminea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7.5 2-pyrone-4,6-dicarboxylate synthesis Pseudomonas straminea
8.5
-
 2-pyrone-4,6-dicarboxylate hydrolysis Pseudomonas straminea

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas straminea isolelectric focusing at 6°C, LKB 8101 column pH 3.5-10.0
-
 5.49