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Literature summary for 3.1.1.54 extracted from

  • Suetfeld, R.; Towers, G.H.N.
    5-(4-Acetoxy-1-butynyl)-2,2'-bithiophene:acetate esterase from Tagetes patula (1982), Phytochemistry, 21, 277-279.
No PubMed abstract available
Search for more literature for 3.1.1.54

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin activity is affected considerably, 5.0 mg/ml prevents substrate polymerization Tagetes patula

General Stability

General Stability Organism
after 6 months of repeated thawing and freezing about 50% of the esterase activity disappears Tagetes patula

Inhibitors

Inhibitors Comment Organism Structure
bovine serum albumin BSA forms a weak conjugate with the substrate which inhibits the enzyme competitively Tagetes patula
additional information ascorbic acid, KCN at 0.001-100 mM, cysteine, glutathione, dithiothreitol, 5,5'-dithiobis(2-nitrobenzoate), 4-chloromercuribenzoate at 0.001-1 mM are no inhibitors Tagetes patula

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 5-(4-Acetoxybut-1-ynyl)-2,2'-bithiophene pH 8.0, 30°C Tagetes patula

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 gel filtration Tagetes patula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-(4-acetoxybut-1-ynyl)-2,2'-bithiophene + H2O Tagetes patula enzyme involved in the transformation of an acetylenic compound 5-(4-hydroxy-but-1-ynyl)-2,2'-bithiophene + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Tagetes patula
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Tagetes patula

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Tagetes patula
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0156
-
-
 Tagetes patula

Storage Stability

Storage Stability Organism
-20°C, purified enzyme can be stored for more than 3 months without a marked loss of activity Tagetes patula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-(4-acetoxybut-1-ynyl)-2,2'-bithiophene + H2O
-
 Tagetes patula 5-(4-hydroxy-but-1-ynyl)-2,2'-bithiophene + acetate
-
 ?
5-(4-acetoxybut-1-ynyl)-2,2'-bithiophene + H2O enzyme involved in the transformation of an acetylenic compound Tagetes patula 5-(4-hydroxy-but-1-ynyl)-2,2'-bithiophene + acetate
-
 ?
additional information none of various acetate esters as well as other naturally occuring esters like chlorogenic acid or p-coumaroyl-CoA are hydrolysed by the enzyme Tagetes patula ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 preparation heated for 5 min is inactive Tagetes patula

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Tagetes patula