General Stability | Organism |
---|---|
-20°C, in absence of glycerol, the enzyme loses all of its activity during the freezing and thawing process | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3(cis,cis-7,10)hexadecadienyl-4-hydroxy-2-butenolide | - |
Mus musculus | |
Ca2+ | - |
Mus musculus | |
Co2+ | - |
Mus musculus | |
Cu2+ | - |
Mus musculus | |
Fe2+ | - |
Mus musculus | |
Fe3+ | partial | Mus musculus | |
Hg2+ | - |
Mus musculus | |
Zn2+ | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
lysophosphatidylcholine | substrate concentration between 0.015-0.2 mM | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
x * 27000, lysophospholipase I, SDS-PAGE | Mus musculus |
28000 | - |
x * 28000, lysophospholipase II, SDS-PAGE | Mus musculus |
30000 | - |
lysophospholipase I, gel filtration | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
lysophospholipase I and II | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
P-388D1 cell | macrophage-like | Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.34 | - |
- |
Mus musculus |
Storage Stability | Organism |
---|---|
-20°C, enzyme concentration: 0.01-0.025 mg/ml, 60-70% glycerol, stable for several months | Mus musculus |
4°C, stable for several weeks | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lysophosphatidylcholine + H2O | - |
Mus musculus | glycerophosphorylcholine + unesterified fatty acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, lysophospholipase II, SDS-PAGE | Mus musculus |
? | x * 27000, lysophospholipase I, SDS-PAGE | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | - |
Mus musculus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | unstable below pH 6.0 and above pH 8.5 | Mus musculus |