Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | minimizes loss of activity in presence of sulfhydryl reagent | Hordeum vulgare |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | modest | Hordeum vulgare | |
Cu2+ | little effect | Hordeum vulgare | |
Hg2+ | little effect | Hordeum vulgare | |
iodoacetamide | - |
Hordeum vulgare | |
NEM | little effect | Hordeum vulgare | |
PCMB | - |
Hordeum vulgare | |
PMSF | little effect | Hordeum vulgare |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
2-lysophosphatidylcholine | - |
Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, PLP I, SDS-PAGE | Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Hordeum vulgare | the function of the enzyme is directed to the disposal of the starch inclusion lipids during their release from the starch matrix during germination | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
var. Advance, at least 3 enzymatic forms: PLP I, PLP II and PLP II' | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | glycoprotein | Hordeum vulgare |
side-chain modification | 10-12% carbohydrate | Hordeum vulgare |
Purification (Comment) | Organism |
---|---|
- |
Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Hordeum vulgare | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
51 | - |
- |
Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-palmitoyl-sn-glycero-3-phosphocholine + H2O | - |
Hordeum vulgare | palmitic acid + glycero-3-phosphocholine | - |
? | |
2-lysophosphatidylcholine + H2O | - |
Hordeum vulgare | glycerophosphorylcholine + a carboxylate | - |
? | |
additional information | the function of the enzyme is directed to the disposal of the starch inclusion lipids during their release from the starch matrix during germination | Hordeum vulgare | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, PLP I, SDS-PAGE | Hordeum vulgare |
Synonyms | Comment | Organism |
---|---|---|
LPL | - |
Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
Tris buffer | Hordeum vulgare |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | pH 5.0: about 40% of maximal activity, pH 9.0: about 50% of maximal activity | Hordeum vulgare |