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Literature summary for 3.1.1.5 extracted from

  • Kovacic, F.; Granzin, J.; Wilhelm, S.; Kojic-Prodic, B.; Batra-Safferling, R.; Jaeger, K.E.
    Structural and functional characterisation of TesA - a novel lysophospholipase A from Pseudomonas aeruginosa (2013), PLoS ONE, 8, e69125.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 20% (w/v) PEG 3350, 50 mM sodium-citrate buffer, pH 4.5 Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
D17S/L162R the mutant shows 2.2fold increased thioesterase activity compared to the wild type Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.78
-
 1-lauryl-glycerophosphocholine at pH 8.0 and 37°C Pseudomonas aeruginosa
2.04
-
 1-palmitoyl-glycerophosphocholine at pH 8.0 and 37°C Pseudomonas aeruginosa
7.22
-
 1-hexyl-glycerophosphocholine at pH 8.0 and 37°C Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 x * 20000, SDS-PAGE Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-hexyl-glycerophosphocholine + H2O
-
 Pseudomonas aeruginosa hexanoate + glycerophosphocholine
-
 ?
1-lauryl-glycerophosphocholine + H2O
-
 Pseudomonas aeruginosa laurate + glycerophosphocholine
-
 ?
1-oleoyl-glycerophosphocholine + H2O
-
 Pseudomonas aeruginosa oleate + glycerophosphocholine
-
 ?
1-palmitoyl-glycerophosphocholine + H2O
-
 Pseudomonas aeruginosa palmitate + glycerophosphocholine
-
 ?
1-stearoyl-glycerophosphocholine + H2O
-
 Pseudomonas aeruginosa stearate + glycerophosphocholine
-
 ?

Subunits

Subunits Comment Organism
? x * 20000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
lysophospholipase A
-
 Pseudomonas aeruginosa
TesA
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
921.1
-
 1-palmitoyl-glycerophosphocholine at pH 8.0 and 37°C Pseudomonas aeruginosa
1016
-
 1-hexyl-glycerophosphocholine at pH 8.0 and 37°C Pseudomonas aeruginosa
1975
-
 1-lauryl-glycerophosphocholine at pH 8.0 and 37°C Pseudomonas aeruginosa

General Information

General Information Comment Organism
metabolism the enzyme exerts important functions in the homeostasis of phospholipids or detoxification of lysophospholipids Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
110
-
 1-hexyl-glycerophosphocholine at pH 8.0 and 37°C Pseudomonas aeruginosa
450
-
 1-palmitoyl-glycerophosphocholine at pH 8.0 and 37°C Pseudomonas aeruginosa
1100
-
 1-lauryl-glycerophosphocholine at pH 8.0 and 37°C Pseudomonas aeruginosa