Cloned (Comment) | Organism |
---|---|
expressed in Arabidopsis thaliana seedlings | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Arabidopsis thaliana | 5783 | - |
peroxisome | - |
Arabidopsis thaliana | 5777 | - |
plasma membrane | - |
Arabidopsis thaliana | 5886 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, SDS-PAGE | Arabidopsis thaliana |
40900 | - |
x * 40900, His-tagged enzyme, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | acyl-CoA-binding protein 2 can bind both lysophosphatidylcholine and lysophospholipase 2 to promote the degradation of lysophosphatidylcholine in response to Cd2+-induced oxidative stress | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
ecotype Col-0 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
silique | - |
Arabidopsis thaliana | - |
stem | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-palmitoyl-2-lysophosphatidylcholine + H2O | - |
Arabidopsis thaliana | palmitate + glycerophosphocholine | - |
? | |
additional information | acyl-CoA-binding protein 2 can bind both lysophosphatidylcholine and lysophospholipase 2 to promote the degradation of lysophosphatidylcholine in response to Cd2+-induced oxidative stress | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE | Arabidopsis thaliana |
? | x * 40900, His-tagged enzyme, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
lysophospholipase 2 | - |
Arabidopsis thaliana |
lysoPL2 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
33 | - |
- |
Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | 43 | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
in Tris-HCl buffer | Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | lysoPL2 mRNA expression in shoots is up-regulated by Zn2+, and H2O2 (but not by Pb2+, Cd2+ and Cu2+), while roots are only up-regulated by H2O2 | up |
General Information | Comment | Organism |
---|---|---|
malfunction | lysoPL2 knockout mutants show enhanced sensitivity to Zn2+ and H2O2 in comparison to wild type | Arabidopsis thaliana |
physiological function | lysophospholipase 2 can effectively remove lysophosphatidylcholine and detoxify the peroxidized membrane. Lysophospholipase 2-overexpressing Arabidopsis is more tolerant to H2O2 and Cd2+ than wild type, suggesting involvement lysophospholipase-2 in phospholipid repair following lipid peroxidation arising from metal-induced stress. The enzyme confers protection during oxidative stress | Arabidopsis thaliana |