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Literature summary for 3.1.1.5 extracted from
- Purification and characterization of phospholipase B from Candida utilis (2006), Biosci. Biotechnol. Biochem., 70, 377-386.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe3+ | at pH 3.0 | Cyberlindnera jadinii |  |
| Hg2+ | at pH 3.0 | Cyberlindnera jadinii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | strong activation at pH 8.0, optimal concentration is 20 mM | Cyberlindnera jadinii | |
| Co2+ | pH 8.0, activates | Cyberlindnera jadinii | |
| Cu2+ | pH 8.0, activates | Cyberlindnera jadinii | |
| Fe2+ | pH 8.0, activates | Cyberlindnera jadinii | |
| Fe3+ | pH 8.0, activates | Cyberlindnera jadinii | |
| Mn2+ | pH 8.0, activates | Cyberlindnera jadinii | |
| additional information | activity at pH 3.0 is independent on metal ions | Cyberlindnera jadinii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyberlindnera jadinii | Q7Z8L3 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Cyberlindnera jadinii |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cyberlindnera jadinii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Cyberlindnera jadinii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28.72 | - |
- |
Cyberlindnera jadinii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-lysophosphatidylcholine + H2O | - |
Cyberlindnera jadinii | glycerophosphocholine + a fatty acid | - |
? | |
| lysophosphatidylethanolamine + H2O | - |
Cyberlindnera jadinii | fatty acid + glycerylphosphoethanolamine | - |
? | |
| phosphatidic acid + H2O | - |
Cyberlindnera jadinii | ? | - |
? | |
| phosphatidylcholine + H2O | - |
Cyberlindnera jadinii | lysophosphatidylcholine + glycerophosphorylcholine + a fatty acid | - |
? | |
| phosphatidylethanolamine + H2O | - |
Cyberlindnera jadinii | lysophosphatidylethanolamine + glycerophosphorylethanolamine + a fatty acid | - |
? | |
| phosphatidylinositol + H2O | - |
Cyberlindnera jadinii | lysophophatidylinositol + a carboxylate | - |
? | |
| phosphatidylserine + H2O | - |
Cyberlindnera jadinii | lysophosphatidylserine + glycerophosphorylserine + a fatty acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 90000-110000, SDS-PAGE | Cyberlindnera jadinii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
at pH 3.0 | Cyberlindnera jadinii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
complete loss of activity after incubation above 80°C | Cyberlindnera jadinii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
two pH-optima: pH 3.0 and pH 7.5 | Cyberlindnera jadinii |
| 7.5 | - |
two pH-optima: pH 3.0 and pH 7.5 | Cyberlindnera jadinii |
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Release 2023.1 (January 2023)
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