Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 10 mM, 50% inhibition of phospholipase activity | Cryptococcus neoformans | |
Fe3+ | 1 mM, 95% inhibition of phospholipase activity, 80% inhibition of lysophospholipase activity | Cryptococcus neoformans | |
palmitoylcarnitine | 10 mM, 70% inhibition of phospholipase activity | Cryptococcus neoformans | |
SDS | 0.05%, complete inhibition of phospholipase activity | Cryptococcus neoformans | |
Triton X-100 | 50% inhibition of lysophospholipase activity | Cryptococcus neoformans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.092 | - |
1-palmitoyl-lysophosphatidylcholine | 37°C, pH 4.0 | Cryptococcus neoformans | |
0.19 | - |
1,2-dipalmitoylphosphatidylcholine | 37°C, pH 4.0 | Cryptococcus neoformans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Cryptococcus neoformans | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | 232000 | native PAGE | Cryptococcus neoformans |
160000 | 180000 | gel filtration | Cryptococcus neoformans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-lysophosphatidylcholine + H2O | Cryptococcus neoformans | - |
glycerophosphocholine + a carboxylate | - |
? | |
phosphatidylcholine + H2O | Cryptococcus neoformans | - |
lysophosphatidylcholine + a carboxylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cryptococcus neoformans | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Cryptococcus neoformans |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, phenyl-Sepharose, Uno Q-1, Superose 12 HR, Superdex 200 | Cryptococcus neoformans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.5 | - |
hydrolysis of 1,2-dipalmitoylphosphatidylcholine | Cryptococcus neoformans |
559 | - |
hydrolysis of 1-palmitoyl-lysophosphatidylcholine | Cryptococcus neoformans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dioleoylphosphatidylcholine + H2O | 129% of activity with 1,2-dipalmitoylphosphatidylcholine | Cryptococcus neoformans | monooleoyl-lysophosphatidylcholine + oleic acid | - |
? | |
1,2-dioleoylphosphatidylethanolamine + H2O | - |
Cryptococcus neoformans | monooleoyl-lysophosphatidylethanolamine + oleic acid | 33.5% of activity with 1,2-dipalmitoylphosphatidylcholine | ? | |
1,2-dioleoylphosphatidylserine + H2O | - |
Cryptococcus neoformans | monooleoyl-lysophosphatidylserine + oleic acid | 22.5% of activity with 1,2-dipalmitoylphosphatidylcholine | ? | |
1,2-dipalmitoyl-sn-glycero-3-phosphocholine + H2O | - |
Cryptococcus neoformans | 1-palmitoyl-sn-glycero-3-phosphocholine + palmitate | - |
? | |
1,2-dipalmitoylphosphatidylcholine + H2O | - |
Cryptococcus neoformans | ? | - |
? | |
1-oleoyl-lysophosphatidylcholine + H2O | 194% of activity with 1-palmitoyl-lysophosphatidylcholine | Cryptococcus neoformans | glycerophosphorylcholine + oleic acid | - |
? | |
1-palmitoyl-2-oleoylphosphatidylcholine + H2O | 36% of activity with 1,2-dipalmitoylphosphatidylcholine | Cryptococcus neoformans | lysophosphatidylcholine + ? | - |
? | |
1-palmitoyl-lysophosphatidylcholine + H2O | - |
Cryptococcus neoformans | glycerophosphocholine + palmitic acid | - |
? | |
1-palmitoyl-lysophosphatidylethanolamine + H2O | 29% of activity with 1-palmitoyl-lysophosphatidylcholine | Cryptococcus neoformans | glycerophosphatidylethanolamine + palmitic acid | - |
? | |
1-palmitoyl-sn-glycero-3-phosphoserine + H2O | 3.8% of activity with 1-palmitoyl-lysophosphatidylcholine | Cryptococcus neoformans | glycerophosphoserine + palmitic acid | - |
? | |
2-lysophosphatidylcholine + H2O | - |
Cryptococcus neoformans | glycerophosphocholine + a carboxylate | - |
? | |
lysophosphatidylinositol + H2O | 78% of activity with 1-palmitoyl-lysophosphatidylcholine | Cryptococcus neoformans | glycerophosphatidylinositol + a carboxylate | - |
? | |
phosphatidylcholine + H2O | - |
Cryptococcus neoformans | lysophosphatidylcholine + a carboxylate | - |
? | |
phosphatidylglycerol + H2O | 94.2% of activity with 1,2-dipalmitoylphosphatidylcholine | Cryptococcus neoformans | lysophosphatidylglycerol + a carboxylate | - |
? | |
phosphatidylinositol + H2O | 15.6% of activity with 1,2-dipalmitoylphosphatidylcholine | Cryptococcus neoformans | lysophophatidylinositol + a carboxylate | - |
? | |
platelet activating factor + H2O | 8.9% of activity with 1,2-dipalmitoylphosphatidylcholine | Cryptococcus neoformans | platelet activating factor + a carboxylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000-90000, SDS-PAGE | Cryptococcus neoformans |
Synonyms | Comment | Organism |
---|---|---|
phospholipase B | - |
Cryptococcus neoformans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
lysophospholipase activity | Cryptococcus neoformans |
37 | - |
phospholipase activity | Cryptococcus neoformans |
40 | - |
transacylase activity | Cryptococcus neoformans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
45% loss of lysophospholipase and transacylase activity, complete loss of phospholipase activity | Cryptococcus neoformans |
60 | - |
complete loss of lysophospholipase and transacylase activity | Cryptococcus neoformans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
hydrolysis of phosphatidylcholin and lysophosphatidylcholin | Cryptococcus neoformans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 6 | approx. 50% of maximal phospholipase activity at pH 3.0 and pH 5.0, respectively | Cryptococcus neoformans |
2.5 | 7 | 50-60% of maximal lysophospholipase activity at pH 3.0 and pH 5.5, respectively | Cryptococcus neoformans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cryptococcus neoformans | 2D-electrophoresis | - |
3.5 |