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Literature summary for 3.1.1.47 extracted from

  • Yasuda, K.; Okumura, T.; Okada, H.; Nakajima, T.; Aoki, J.; Arai, H.; Inoue, K.; Nishizawa, M.; Ito, S.; Kanzaki, H.
    Platelet-activating factor acetylhydrolase isoforms I and II in human uterus. Comparisons with pregnant uterus and myoma (2001), Biol. Reprod., 64, 339-344.
    View publication on PubMed
Search for more literature for 3.1.1.47

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol at least 2 isozymes I and II Homo sapiens 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 1 * 29000, alpha1-subunit, + 1 * 30000, alpha2-subunit, + 1 * 45000, beta-subunit, fetal brain enzyme, SDS-PAGE, 1 * 20000-25000, alpha1-subunit, + 1 * 30000, alpha2-subunit, + 1 * 45000, beta-subunit, kidney and uterus enzyme, SDS-PAGE Homo sapiens
30000
-
 1 * 29000, alpha1-subunit, + 1 * 30000, alpha2-subunit, + 1 * 45000, beta-subunit, fetal brain enzyme, SDS-PAGE, 1 * 20000-25000, alpha1-subunit, + 1 * 30000, alpha2-subunit, + 1 * 45000, beta-subunit, kidney and uterus enzyme, SDS-PAGE Homo sapiens
45000
-
 1 * 29000, alpha1-subunit, + 1 * 30000, alpha2-subunit, + 1 * 45000, beta-subunit, fetal brain enzyme, SDS-PAGE, 1 * 20000-25000, alpha1-subunit, + 1 * 30000, alpha2-subunit, + 1 * 45000, beta-subunit, kidney and uterus enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + H2O Homo sapiens i.e. platelet-activation factor 1-hexadecyl-sn-glycero-3-phosphocholine + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain fetal Homo sapiens
-
kidney
-
 Homo sapiens
-
myoma cell at least 2 isozymes I and II, increased amount of isozyme I beta-subunit Homo sapiens
-
myometrium at least 2 isozymes I and II Homo sapiens
-
uterus isozyme I activity is lower in pregnant uterus compared to the nonpregnant one Homo sapiens
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000008
-
 cytosolic fraction, nonpregnant uterus Homo sapiens
0.000073
-
 cytosolic fraction, pregnant uterine myometrium Homo sapiens
0.000221
-
 cytosolic fraction, myoma Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + H2O i.e. platelet-activation factor Homo sapiens 1-hexadecyl-sn-glycero-3-phosphocholine + acetate
-
 ?
2-hexadecyl-1-alkyl-sn-glycero-3-phosphocholine + H2O i.e. platelet-activation factor Homo sapiens 1-hexadecyl-sn-glycero-3-phosphocholine + acetate
-
 ?

Subunits

Subunits Comment Organism
More isozyme I is a trimer of 2 alpha-subunits and one beta-subunit, isozyme II is a monomer Homo sapiens
trimer 1 * 29000, alpha1-subunit, + 1 * 30000, alpha2-subunit, + 1 * 45000, beta-subunit, fetal brain enzyme, SDS-PAGE, 1 * 20000-25000, alpha1-subunit, + 1 * 30000, alpha2-subunit, + 1 * 45000, beta-subunit, kidney and uterus enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
PAF acetylhydrolase
-
 Homo sapiens
PAF-AH
-
 Homo sapiens
platelet-activating factor acetylhydrolase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens