Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-O-hexadecanoyl-sn-glycero-3-phosphocholine | competitive inhibition of acetylhydrolase activity | Homo sapiens | |
4-(2-aminoethyl)benzenesulfonyl fluoride | i.e. pefabloc, inhibits both acetylhydrolase and transacetylase activities completely at 0.05 mM for the native enzyme, and at 1 mM for the recombinant enzyme | Homo sapiens | |
diisopropylfluorophosphate | inhibits both acetylhydrolase and transacetylase activities completely at 20 mM for the native and the recombinant enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the recombinant enzyme shows allosteric kinetics in the transacetylation reaction | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion, plasma | Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecanoyl-sn-glycero-3-phosphocholine | Homo sapiens | transacetylation | 1-hexadecyl-sn-glycero-3-phosphocholine + 2-acetyl-1-hexadecanoyl-sn-glycero-3-phosphocholine | - |
? | |
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-sn-glycero-3-phosphocholine | Homo sapiens | the transacetylase activity may acetylate extracellular lyso-PAF into biologically active PAF | 1-hexadecyl-sn-glycero-3-phosphocholine + 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine | - |
? | |
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + H2O | Homo sapiens | i.e. platelet-activation factor, biological inactivation by deacetylation | 1-hexadecyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
additional information | Homo sapiens | the enzyme is associated to low-density lipoprotein LDL particles, both enzyme activities, acetylhydrolase and transacetylase, decrease during LDL oxidation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate | the enzyme possesses both acetylhydrolase and transacetylase activities which remove PAF and its ether-linked analogues from LDL-particles upon LDL oxidation | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
plasma | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.015 | - |
transacetylase activity | Homo sapiens |
0.022 | - |
acetylhydrolase activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecanoyl-sn-glycero-3-phosphocholine | transacetylase activity | Homo sapiens | 1-hexadecyl-sn-glycero-3-phosphocholine + 2-acetyl-1-hexadecanoyl-sn-glycero-3-phosphocholine | - |
? | |
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecanoyl-sn-glycero-3-phosphocholine | transacetylation | Homo sapiens | 1-hexadecyl-sn-glycero-3-phosphocholine + 2-acetyl-1-hexadecanoyl-sn-glycero-3-phosphocholine | - |
? | |
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-sn-glycero-3-phosphocholine | transacetylase activity | Homo sapiens | 1-hexadecyl-sn-glycero-3-phosphocholine + 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine | - |
? | |
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-sn-glycero-3-phosphocholine | the transacetylase activity may acetylate extracellular lyso-PAF into biologically active PAF | Homo sapiens | 1-hexadecyl-sn-glycero-3-phosphocholine + 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine | - |
? | |
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + H2O | i.e. platelet-activation factor PAF | Homo sapiens | 1-hexadecyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + H2O | i.e. platelet-activation factor, biological inactivation by deacetylation | Homo sapiens | 1-hexadecyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
2-butanoyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-sn-glycero-3-phosphocholine | transacetylase activity, 40% of the activity with PAF | Homo sapiens | 1-hexadecyl-sn-glycero-3-phosphocholine + 2-butanoyl-1-hexadecyl-sn-glycero-3-phosphocholine | - |
? | |
2-propionyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-sn-glycero-3-phosphocholine | transacetylase activity, 73% of the activity with PAF | Homo sapiens | 1-hexadecyl-sn-glycero-3-phosphocholine + 2-propionyl-1-hexadecyl-sn-glycero-3-phosphocholine | - |
? | |
2-valeroyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-sn-glycero-3-phosphocholine | transacetylase activity, 17% of the activity with PAF | Homo sapiens | 1-hexadecyl-sn-glycero-3-phosphocholine + 2-valeroyl-1-hexadecyl-sn-glycero-3-phosphocholine | - |
? | |
additional information | cholesteryl acetate is inactive as acetyl donor in the transacetylation reaction | Homo sapiens | ? | - |
? | |
additional information | the enzyme is associated to low-density lipoprotein LDL particles, both enzyme activities, acetylhydrolase and transacetylase, decrease during LDL oxidation | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAF acetylhydrolase | - |
Homo sapiens |
PAF-AH | - |
Homo sapiens |
platelet-activating factor acetylhydrolase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
60 min, complete inactivation of both activities, acetylhydrolase and transacetylase | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |