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Literature summary for 3.1.1.45 extracted from
- Dienelactone hydrolase from Pseudomonas cepacia (1993), J. Bacteriol., 175, 2994-3001.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | no inactivation | Burkholderia cepacia |  |
| additional information | no inhibition by trans-4-carboxymethylenebut-2-en-4-olide, (+)-muconolactone, (+)-4-fluoromuconolactone | Burkholderia cepacia | |
| p-chloromercuribenzoate | not inhibitory | Burkholderia cepacia | |
| succinate | reversible inhibitory effect | Burkholderia cepacia | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.59 | - |
cis-4-carboxymethylenebut-2-en-4-olide | - |
Burkholderia cepacia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
gel filtration | Burkholderia cepacia |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Burkholderia cepacia | natural function is not in the degradation of haloaromatic compounds, the normal substrate might well be one of the more complex natural compounds with a dienelactone structure, or it could also be an intermediate of some other metabolic pathway | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia cepacia | - |
type II DLH: cis-dienelactone hydrolase | - |
| Burkholderia cepacia | - |
Pseudomonas cepacia | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Burkholderia cepacia |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 217 | - |
- |
Burkholderia cepacia |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 5 months, specific activity decreases from 217 to 65 U/mg | Burkholderia cepacia |
| 20°C, sodium succinate, pH 5, perfectly stable during incubation overnight | Burkholderia cepacia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis-4-carboxymethylenebut-2-en-4-olide + H2O | - |
Burkholderia cepacia | 4-oxohex-2-enedioate | - |
? | |
| additional information | muconolactone is not hydrolyzed to a significant extent | Burkholderia cepacia | ? | - |
? | |
| additional information | natural function is not in the degradation of haloaromatic compounds, the normal substrate might well be one of the more complex natural compounds with a dienelactone structure, or it could also be an intermediate of some other metabolic pathway | Burkholderia cepacia | ? | - |
? | |
| trans-4-carboxymethylenebut-2-en-4-olide + H2O | not a substrate to a significant extent | Burkholderia cepacia | 4-oxohex-2-enedioate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1* 32400, SDS-PAGE | Burkholderia cepacia |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 817 | - |
cis-4-carboxymethylenebut-2-en-4-olide | - |
Burkholderia cepacia | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Burkholderia cepacia |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8.1 | - |
Burkholderia cepacia |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
most stable around, decreasing faster towards lower than towards higher pH values | Burkholderia cepacia |
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Release 2023.1 (January 2023)
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