Crystallization (Comment) | Organism |
---|---|
- |
Pseudomonas knackmussii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuribenzoate | - |
Cupriavidus necator | |
p-chloromercuribenzoate | - |
Pseudomonas knackmussii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-carboxymethylenebut-2-en-4-olide + H2O | Pseudomonas knackmussii | the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | - |
Pseudomonas cepacia | - |
Cupriavidus necator | - |
Alcaligenes eutrophus | - |
Pseudomonas knackmussii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
3-chlorobenzoate-grown cells | Pseudomonas knackmussii |
Storage Stability | Organism |
---|---|
4°C, converted to microcrystals, ammonium sulfate to 30% saturation, stable for more than 1 year | Pseudomonas knackmussii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-carboxymethylenebut-2-en-4-olide + H2O | the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds | Pseudomonas knackmussii | ? | - |
? | |
cis-4-carboxymethylenebut-2-en-4-olide + H2O | - |
Cupriavidus necator | 4-oxohex-2-enedioate | - |
? | |
cis-4-carboxymethylenebut-2-en-4-olide + H2O | - |
Burkholderia cepacia | 4-oxohex-2-enedioate | - |
? | |
cis-4-carboxymethylenebut-2-en-4-olide + H2O | - |
Pseudomonas knackmussii | 4-oxohex-2-enedioate | - |
ir | |
trans-4-carboxymethylenebut-2-en-4-olide + H2O | - |
Cupriavidus necator | 4-oxohex-2-enedioate | - |
? | |
trans-4-carboxymethylenebut-2-en-4-olide + H2O | - |
Pseudomonas knackmussii | 4-oxohex-2-enedioate | - |
ir |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Burkholderia cepacia |
monomer | 1* 28000, SDS-PAGE with mercaptoethanol | Pseudomonas knackmussii |