Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.45 extracted from

  • Porter, J.L.; Collyer, C.A.; Ollis, D.L.
    Compensatory stabilizing role of surface mutations during the directed evolution of dienelactone hydrolase for enhanced activity (2015), Protein J., 34, 82-89 .
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information the surface mutations Q110L, Y137C and N154D do not influence the kinetic properties. Mutations Q110L and N154D have stabilizing effects, the Y137C mutation alone is destabilizing. The three mutations together increase the melting temperature by 3.4 degrees Pseudomonas knackmussii
Q35H/F38L/C123S/Y137C/Y145C/E199G/S208G/G211D mutations do not influence the kinetic properties Pseudomonas knackmussii
Q35H/F38L/C123S/Y145C/E199G/S208G/G211D mutations do not influence the kinetic properties Pseudomonas knackmussii
Q35H/F38L/C123S/Y145C/N154D/E199G/S208G/G211D mutations do not influence the kinetic properties Pseudomonas knackmussii
Q35H/F38L/Q110L/C123S/Y137C/Y145C/N154D/E199G/S208G/G211D mutations do not influence the kinetic properties Pseudomonas knackmussii
Q35H/F38L/Q110L/C123S/Y145C/E199G/S208G/G211D mutations do not influence the kinetic properties Pseudomonas knackmussii
Q35H/F38L/Q110L/C123S/Y145C/N154D/E199G/S208G/G211D mutations do not influence the kinetic properties Pseudomonas knackmussii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
4-nitrophenyl acetate mutant Q35H/F38L/C123S/Y145C/N154D/E199G/S208G/G211D, pH 7.5, 25°C Pseudomonas knackmussii
0.013
-
4-nitrophenyl acetate mutant Q35H/F38L/C123S/Y137C/Y145C/E199G/S208G/G211D, pH 7.5, 25°C Pseudomonas knackmussii
0.013
-
4-nitrophenyl acetate mutant Q35H/F38L/C123S/Y145C/E199G/S208G/G211D, pH 7.5, 25°C Pseudomonas knackmussii
0.013
-
4-nitrophenyl acetate mutant Q35H/F38L/Q110L/C123S/Y145C/N154D/E199G/S208G/G211D, pH 7.5, 25°C Pseudomonas knackmussii
0.014
-
4-nitrophenyl acetate mutant Q35H/F38L/Q110L/C123S/Y137C/Y145C/N154D/E199G/S208G/G211D, pH 7.5, 25°C Pseudomonas knackmussii
0.014
-
4-nitrophenyl acetate mutant Q35H/F38L/Q110L/C123S/Y145C/E199G/S208G/G211D, pH 7.5, 25°C Pseudomonas knackmussii

Organism

Organism UniProt Comment Textmining
Pseudomonas knackmussii P0A115
-
-
Pseudomonas knackmussii DSM 6978 P0A115
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
Pseudomonas knackmussii 4-nitrophenol + acetate
-
?
4-nitrophenyl acetate + H2O
-
Pseudomonas knackmussii DSM 6978 4-nitrophenol + acetate
-
?

Synonyms

Synonyms Comment Organism
ClcD
-
Pseudomonas knackmussii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
51.5
-
melting temperature, mutant Q35H/F38L/C123S/Y145C/E199G/S208G/G211D Pseudomonas knackmussii
54.9
-
melting temperature, mutant Q35H/F38L/Q110L/C123S/Y137C/Y145C/N154D/E199G/S208G/G211D Pseudomonas knackmussii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.57
-
4-nitrophenyl acetate mutant Q35H/F38L/C123S/Y137C/Y145C/E199G/S208G/G211D, pH 7.5, 25°C Pseudomonas knackmussii
0.58
-
4-nitrophenyl acetate mutant Q35H/F38L/C123S/Y145C/N154D/E199G/S208G/G211D, pH 7.5, 25°C Pseudomonas knackmussii
0.58
-
4-nitrophenyl acetate mutant Q35H/F38L/Q110L/C123S/Y145C/N154D/E199G/S208G/G211D, pH 7.5, 25°C Pseudomonas knackmussii
0.59
-
4-nitrophenyl acetate mutant Q35H/F38L/C123S/Y145C/E199G/S208G/G211D, pH 7.5, 25°C Pseudomonas knackmussii
0.6
-
4-nitrophenyl acetate mutant Q35H/F38L/Q110L/C123S/Y145C/E199G/S208G/G211D, pH 7.5, 25°C Pseudomonas knackmussii
0.61
-
4-nitrophenyl acetate mutant Q35H/F38L/Q110L/C123S/Y137C/Y145C/N154D/E199G/S208G/G211D, pH 7.5, 25°C Pseudomonas knackmussii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
43
-
4-nitrophenyl acetate mutant Q35H/F38L/C123S/Y137C/Y145C/E199G/S208G/G211D, pH 7.5, 25°C Pseudomonas knackmussii
43
-
4-nitrophenyl acetate mutant Q35H/F38L/Q110L/C123S/Y145C/E199G/S208G/G211D, pH 7.5, 25°C Pseudomonas knackmussii
44
-
4-nitrophenyl acetate mutant Q35H/F38L/Q110L/C123S/Y145C/N154D/E199G/S208G/G211D, pH 7.5, 25°C Pseudomonas knackmussii
45
-
4-nitrophenyl acetate mutant Q35H/F38L/Q110L/C123S/Y137C/Y145C/N154D/E199G/S208G/G211D, pH 7.5, 25°C Pseudomonas knackmussii
46
-
4-nitrophenyl acetate mutant Q35H/F38L/C123S/Y145C/E199G/S208G/G211D, pH 7.5, 25°C Pseudomonas knackmussii
49
-
4-nitrophenyl acetate mutant Q35H/F38L/C123S/Y145C/N154D/E199G/S208G/G211D, pH 7.5, 25°C Pseudomonas knackmussii