Cloned (Comment) | Organism |
---|---|
into pGEM-T Easy and subsequently into pASK-IBA7plus for expression of the protein in Escherichia coli XL10-GOLD cells | Pseudomonas reinekei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | to evaluate the metal dependence of trans-DLH, a strep-tagged protein is purified | Pseudomonas reinekei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Pseudomonas reinekei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.55 | - |
trans-dienelactone | - |
Pseudomonas reinekei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Pseudomonas reinekei | |
Ni2+ | - |
Pseudomonas reinekei | |
Zn2+ | - |
Pseudomonas reinekei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36940 | - |
calculated molecular weight | Pseudomonas reinekei |
37000 | - |
determined by SDS-PAGE | Pseudomonas reinekei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-chloromuconolactone + H2O | Pseudomonas reinekei | - |
maleylacetate + HCl | - |
? | |
trans-dienelactone + H2O | Pseudomonas reinekei | - |
maleylacetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas reinekei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant and wild-type trans-DLH are purified from cell extracts of Escherichia coli XL10-GOLD and Pseudomonas reinekei by subsequent anionic exchange, hydrophobic interaction, gel filtration and anionic exchange chromatography, the strep-tagged recombinant protein is purified using a Strep-Tactin Sepharose column | Pseudomonas reinekei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
29 | - |
strep-tagged protein, contains 1.9 mol of Zn2+, less than 0.1 mol of Mn2+, and 1.1 mol Ni2+ per mol of subunit | Pseudomonas reinekei |
34 | - |
recombinant protein, contains 2.3 mol Zn2+, 0.8 mol Mn2+, and less than 0.1 mol Ni2+ per mol of subunit | Pseudomonas reinekei |
65 | - |
wild-type, contains 2.4 mol Zn2+, 2.0 mol Mn2+, and 1.1 mol Ni2+ per mol of subunit | Pseudomonas reinekei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-chloromuconolactone + H2O | - |
Pseudomonas reinekei | maleylacetate + HCl | - |
? | |
trans-dienelactone + H2O | - |
Pseudomonas reinekei | maleylacetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
trans-dienelactone hydrolase | - |
Pseudomonas reinekei |
trans-DLH | - |
Pseudomonas reinekei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
standard condition | Pseudomonas reinekei |
55 | - |
assay for determination of temperature-dependence of the activity | Pseudomonas reinekei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15100 | - |
trans-dienelactone | recombinant protein | Pseudomonas reinekei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay for determination of pH-dependence of the activity | Pseudomonas reinekei |
7.5 | - |
activity assay | Pseudomonas reinekei |