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Literature summary for 3.1.1.45 extracted from

  • Nikodem, P.; Hecht, V.; Schlomann, M.; Pieper, D.H.
    New bacterial pathway for 4- and 5-chlorosalicylate degradation via 4-chlorocatechol and maleylacetate in Pseudomonas sp. strain MT1 (2003), J. Bacteriol., 185, 6790-6800.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.45

Activating Compound

Activating Compound Comment Organism Structure
chlorocatechols growth on chlorocatechols induce the trans-dienelactone hydrolase activity by 6fold Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 34% remaining activity after 5 min, 1 mM Pseudomonas sp.
EDTA 27% remaining activity after 5 min, 17% remaining activity after 60 min, 1 mM Pseudomonas sp.
Hg2+ 6% remaining activity after 5 min, 1 mM Pseudomonas sp.
additional information no inhibition by 4-chloromercuribenzoate Pseudomonas sp.
o-phenanthroline 55% remaining activity after 5 min, 18% remaining activity after 60 min, 1 mM Pseudomonas sp.
Zn2+ below 5% remaining activity after 5 min, 1 mM Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.48
-
 trans-dienelactone pH 7.5, 25°C Pseudomonas sp.
1.2
-
 4-fluoromuconolactone pH 7.5, 25°C Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 2 * 39000, SDS-PAGE Pseudomonas sp.
85000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas sp. antibiotic protoanemonin is no substrate ?
-
 ?
additional information Pseudomonas sp. MT1 antibiotic protoanemonin is no substrate ?
-
 ?
trans-dienelactone + H2O Pseudomonas sp. degradation of chlorosalicylates via chlorocatechols to 3-oxoadipate via maleylacetate, pathway overview maleylacetate
-
 ?
trans-dienelactone + H2O Pseudomonas sp. MT1 degradation of chlorosalicylates via chlorocatechols to 3-oxoadipate via maleylacetate, pathway overview maleylacetate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 possesses only the trans-substrate isomer-specific enzyme form
-
Pseudomonas sp. MT1
-
 possesses only the trans-substrate isomer-specific enzyme form
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
190
-
 purified enzyme, substrate 4-fluoromuconolactone Pseudomonas sp.
710
-
 purified enzyme, substrate trans-dienelactone Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-fluoromuconolactone + H2O
-
 Pseudomonas sp. maleylacetate + HF
-
 ?
4-fluoromuconolactone + H2O
-
 Pseudomonas sp. MT1 maleylacetate + HF
-
 ?
additional information antibiotic protoanemonin is a poor substrate Pseudomonas sp. ?
-
 ?
additional information antibiotic protoanemonin is no substrate Pseudomonas sp. ?
-
 ?
additional information antibiotic protoanemonin is a poor substrate Pseudomonas sp. MT1 ?
-
 ?
additional information antibiotic protoanemonin is no substrate Pseudomonas sp. MT1 ?
-
 ?
trans-dienelactone + H2O very low activity with the cis-isomer Pseudomonas sp. maleylacetate
-
 ?
trans-dienelactone + H2O degradation of chlorosalicylates via chlorocatechols to 3-oxoadipate via maleylacetate, pathway overview Pseudomonas sp. maleylacetate
-
 ?
trans-dienelactone + H2O very low activity with the cis-isomer Pseudomonas sp. MT1 maleylacetate
-
 ?
trans-dienelactone + H2O degradation of chlorosalicylates via chlorocatechols to 3-oxoadipate via maleylacetate, pathway overview Pseudomonas sp. MT1 maleylacetate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 39000, SDS-PAGE Pseudomonas sp.

Synonyms

Synonyms Comment Organism
cis-dienelactone hydrolase
-
 Pseudomonas sp.
cis-DLH
-
 Pseudomonas sp.
dienelactone hydrolase
-
 Pseudomonas sp.
DLH
-
 Pseudomonas sp.
trans-dienelactone hydrolase
-
 Pseudomonas sp.
trans-DLH
-
 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1630
-
 4-fluoromuconolactone pH 7.5, 25°C Pseudomonas sp.
1633
-
 4-fluoromuconolactone pH 7.5, 25°C Pseudomonas sp.
2650
-
 trans-dienelactone pH 7.5, 25°C Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Pseudomonas sp.