Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.43 extracted from

  • Hossain, M.S.; Tanaka, T.; Takagi, K.; Hayashi, J.; Wakayama, M.
    Purification and characterization of Stenotrophomonas maltophilia-derived l-amino acid ester hydrolase for synthesizing dipeptide, isoleucyl-tryptophan (2018), 3 Biotech, 8, 173 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.43

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, complete inhibition Stenotrophomonas maltophilia
Ca2+ 1 mM, 25% inhibition Stenotrophomonas maltophilia
Cu2+ 1 mM, 25% inhibition Stenotrophomonas maltophilia
Fe2+ 1 mM, 30% inhibition Stenotrophomonas maltophilia
Li+ 1 mM, 30% inhibition Stenotrophomonas maltophilia
phenylmethanesulfonyl fluoride 1 mM, complete inhibition Stenotrophomonas maltophilia

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ 1 mM, 130% of initial activity Stenotrophomonas maltophilia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
300000
-
 gel filtration Stenotrophomonas maltophilia

Organism

Organism UniProt Comment Textmining
Stenotrophomonas maltophilia A0A2Z6IAJ2
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.34
-
 substrate L-isoleucyl methyl ester, pH 7.0, 30°C Stenotrophomonas maltophilia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-alanyl methyl ester + L-tryptophan about 100% of the activity with L-isoleucyl methyl ester Stenotrophomonas maltophilia beta-alanyl-L-tryptophan + methanol
-
 ?
glycyl methyl ester + L-tryptophan about 230% of the activity with L-isoleucyl methyl ester Stenotrophomonas maltophilia glycyl-L-tryptophan + methanol
-
 ?
L-alanyl methyl ester + L-tryptophan about 240% of the activity with L-isoleucyl methyl ester Stenotrophomonas maltophilia L-alanyl-L-tryptophan + methanol
-
 ?
L-arginyl methyl ester + L-tryptophan about 220% of the activity with L-isoleucyl methyl ester Stenotrophomonas maltophilia L-arginyl-L-tryptophan + methanol
-
 ?
L-aspartyl methyl ester + L-tryptophan about 200% of the activity with L-isoleucyl methyl ester Stenotrophomonas maltophilia L-aspartyl-L-tryptophan + methanol
-
 ?
L-isoleucyl methyl ester + L-tryptophan
-
 Stenotrophomonas maltophilia L-isoleucyl-L-tryptophan + methanol
-
 ?
L-proyl methyl ester + L-tryptophan about 240% of the activity with L-isoleucyl methyl ester Stenotrophomonas maltophilia L-prolyl-L-tryptophan + methanol
-
 ?
L-tryptophanyl methyl ester + L-tryptophan
-
 Stenotrophomonas maltophilia L-tryptophanyl-L-tryptophan + methanol
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 70000, SDS-PAGE Stenotrophomonas maltophilia

Synonyms

Synonyms Comment Organism
AEH
-
 Stenotrophomonas maltophilia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Stenotrophomonas maltophilia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 10 min, 40% loss of activity Stenotrophomonas maltophilia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Stenotrophomonas maltophilia