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Literature summary for 3.1.1.42 extracted from

  • Negrel, J.; Javelle, F.; Morandi, D.; Lucchi, G.
    Characterization and purification of a bacterial chlorogenic acid esterase detected during the extraction of chlorogenic acid from arbuscular mycorrhizal tomato roots (2016), Plant Physiol. Biochem., 109, 308-318 .
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
chlorogenate pH not specified in the publication, temperature not specified in the publication bacterium

Organic Solvent Stability

Organic Solvent Comment Organism
Methanol esterase is remarkably stable in the presence of methanol, rapid formation of methyl caffeate occurrs when its activity is measured in aqueous solutions containing 10-60% methanol bacterium

Organism

Organism UniProt Comment Textmining
bacterium
-
Gram-negative bacterium isolated from the roots of tomato plants inoculated with the arbuscular mycorrhizal fungus Rhizophagus irregularis
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-caffeoylquinic acid + H2O
-
bacterium caffeate + quinate
-
?
4-caffeoylquinic acid + H2O
-
bacterium caffeate + quinate
-
?
caffeoylshikimate + H2O
-
bacterium caffeate + shikimate
-
?
chlorogenate + H2O
-
bacterium caffeate + quinate
-
?
methyl caffeate + H2O
-
bacterium methanol + caffeate
-
?
methyl ferulate + H2O
-
bacterium methanol + ferulate
-
?

Subunits

Subunits Comment Organism
? x * 61000, SDS-PAGE bacterium

pI Value

Organism Comment pI Value Maximum pI Value
bacterium isoelectric focusing
-
7.8

Expression

Organism Comment Expression
bacterium expression is induced in the presence of hydroxycinnamic acids as sole carbon source up

General Information

General Information Comment Organism
physiological function the bacterium is able to grow using chlorogenic acid as sole carbon source bacterium