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Literature summary for 3.1.1.42 extracted from

  • Wang, X.; Bai, Y.; Cai, Y.; Zheng, X.
    Biochemical characteristics of three feruloyl esterases with a broad substrate spectrum from Bacillus amyloliquefaciens H47 (2017), Process Biochem., 53, 109-115 .
No PubMed abstract available
Search for more literature for 3.1.1.42

Activating Compound

Activating Compound Comment Organism Structure
EDTA 1 mM, 108% of initial activity Bacillus amyloliquefaciens
EDTA 1 mM, 111% of initial activity Bacillus amyloliquefaciens
EDTA 1 mM, 62.7% of initial activity Bacillus amyloliquefaciens

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 1 mM, 93% of initial activity Bacillus amyloliquefaciens
Cu2+ 1 mM, 55.6% of initial activity; 1 mM, 57.6% of initial activity; 1 mM, 8.5% of initial activity Bacillus amyloliquefaciens
Fe2+ 1 mM, 61% of initial activity; 1 mM, 71% of initial activity; 1 mM, 84% of initial activity Bacillus amyloliquefaciens
K+ 1 mM, 88% of initial activity Bacillus amyloliquefaciens
Mg2+ 1 mM, 92% of initial activity; 1 mM, 94% of initial activity Bacillus amyloliquefaciens
Mn2+ 1 mM, 83% of initial activity Bacillus amyloliquefaciens
Na+ 1 mM, 95% of initial activity Bacillus amyloliquefaciens
Ni2+ 1 mM, 15.1% of initial activity; 1 mM, 44.5% of initial activity; 1 mM, 63% of initial activity Bacillus amyloliquefaciens
PMSF 1 mM, 15% of initial activity; 1 mM, 55% of initial activity; 1 mM, 60% of initial activity Bacillus amyloliquefaciens
Zn2+ 1 mM, 14.3% of initial activity; 1 mM, 67.7% of initial activity; 1 mM, 74% of initial activity Bacillus amyloliquefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.51
-
 chlorogenate pH 8.0, 35°C Bacillus amyloliquefaciens
4.79
-
 chlorogenate pH 8.0, 40°C Bacillus amyloliquefaciens
6.06
-
 chlorogenate pH 8.0, 30°C Bacillus amyloliquefaciens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 107% of initial activity Bacillus amyloliquefaciens
Co2+ 1 mM, 115% of initial activity Bacillus amyloliquefaciens
Co2+ 1 mM, 107% of initial activity Bacillus amyloliquefaciens
K+ 1 mM, 115% of initial activity Bacillus amyloliquefaciens
K+ 1 mM, 105% of initial activity Bacillus amyloliquefaciens
Mg2+ 1 mM, 143% of initial activity Bacillus amyloliquefaciens
Mn2+ 1 mM, 113% of initial activity Bacillus amyloliquefaciens
Na+ 1 mM, 120% of initial activity Bacillus amyloliquefaciens
Na+ 1 mM, 151% of initial activity Bacillus amyloliquefaciens

Organism

Organism UniProt Comment Textmining
Bacillus amyloliquefaciens A0A1B1WA13 isoform Fae04, cf. EC 3.1.1.73
-
Bacillus amyloliquefaciens A0A1B1WA14 isoform Fae06, cf. EC 3.1.1.73
-
Bacillus amyloliquefaciens ANW61885 isoform Fae09, cf. EC 3.1.1.73
-
Bacillus amyloliquefaciens H47 A0A1B1WA13 isoform Fae04, cf. EC 3.1.1.73
-
Bacillus amyloliquefaciens H47 A0A1B1WA14 isoform Fae06, cf. EC 3.1.1.73
-
Bacillus amyloliquefaciens H47 ANW61885 isoform Fae09, cf. EC 3.1.1.73
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorogenate + H2O
-
 Bacillus amyloliquefaciens caffeate + quinate
-
 ?
chlorogenate + H2O
-
 Bacillus amyloliquefaciens H47 caffeate + quinate
-
 ?
additional information enzyme additionally acts as feruloyl esterase, cleaving ethyl and methyl esters of hydroxycinnamic acis, reaction of EC 3.1.1.73 Bacillus amyloliquefaciens ?
-
 ?
additional information enzyme additionally acts as feruloyl esterase, cleaving ethyl and methyl esters of hydroxycinnamic acis, reaction of EC 3.1.1.73 Bacillus amyloliquefaciens H47 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE Bacillus amyloliquefaciens
? x * 30000, SDS-PAGE Bacillus amyloliquefaciens
? x * 29000, SDS-PAGE Bacillus amyloliquefaciens

Synonyms

Synonyms Comment Organism
KX091144
-
 Bacillus amyloliquefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Bacillus amyloliquefaciens
35
-
-
 Bacillus amyloliquefaciens
40
-
-
 Bacillus amyloliquefaciens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60
-
 60% residual activity Bacillus amyloliquefaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 complete inactivation Bacillus amyloliquefaciens
60
-
 complete inactivation Bacillus amyloliquefaciens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.52
-
 chlorogenate pH 8.0, 35°C Bacillus amyloliquefaciens
12.06
-
 chlorogenate pH 8.0, 40°C Bacillus amyloliquefaciens
275
-
 chlorogenate pH 8.0, 30°C Bacillus amyloliquefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Bacillus amyloliquefaciens

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 5
-
 Bacillus amyloliquefaciens
8 9
-
 Bacillus amyloliquefaciens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.52
-
 chlorogenate pH 8.0, 40°C Bacillus amyloliquefaciens
4.32
-
 chlorogenate pH 8.0, 35°C Bacillus amyloliquefaciens
45.31
-
 chlorogenate pH 8.0, 30°C Bacillus amyloliquefaciens